Sodium alginate and pectin estimation in raft forming pharmaceuticals by high performance liquid chromatography method

• Autorzy: Hanif Muhammad , Shah Shahid , Rasool Nasir , Abbas Ghulam , Saadullah Malik , Khan Sajid Mehmood , Ahmed Muhammad Masood , Abbas Nazar , Ashfaq Mehran , Iqbal Omeira

Identyfikatory

DOI

10.1556/1326.2020.00765

• Języki publikacji: EN

Abstrakty

EN

The high performance liquid chromatographic (HPLC) method was developed for the combined estimation of sodium alginate and pectin in raft forming pharmaceuticals on C18 column ZORBAX ODS (1.5 cm × 4.6 mm, 5 μm) with UV detection at 378 nm. The assay condition comprised of phosphate buffer pH 7.4 and methanol 60:40% v/v at a flow rate of 1.25 mL/min. The separation of sodium alginate and pectin with good resolution and a retention time less than 8 min was attained. The method was linear over a range of 200–800 μg/mL of sodium alginate and pectin. The regression values obtained from linearity curve of sodium alginate and pectin were 0.9993 and 0.9991, respectively. The retention time of sodium alginate and pectin was 3.931 and 7.470 min, respectively. The percent recovery of sodium alginate and pectin ranged from 94.2–98.5% and 92.1–98.4% respectively. The limit of detection (LOD) and limit of quantification (LOQ) of sodium alginate were found to be 2.443 and 3.129 μg/mL and the LOD and LOQ of pectin were 3.126 and 3.785 μg/mL, respectively. The resolution of sodium alginate and pectin was found in the range of 1.03–1.89 and 1.10–1.91, respectively. This method has been successfully applied to analyze the concentrations of sodium alginate and pectin in raft forming drug delivery systems.

Słowa kluczowe

EN

sodium alginate; pectin; raft; HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2021
• Tom: Vol. 33, no. 2
• Strony: 127--133
• Opis fizyczny: Bibliogr. 13 poz., rys., tab.

Twórcy

autor

Hanif Muhammad

• Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan, Pakistan

autor

Shah Shahid

• Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Faisalabad, Pakistan

autor

Rasool Nasir

• Department of Chemistry, Government College University Faisalabad, Faisalabad, Pakistan

autor

Abbas Ghulam

• Department of Pharmaceutics, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Faisalabad, Pakistan

• https://orcid.org/0000-0002-4531-3911

autor

Saadullah Malik

• Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Faisalabad, Pakistan

autor

Khan Sajid Mehmood

• Faculty of Pharmacy and Alternative Medicines, The Islamia University Bahawalpur, Bahawalpur, Pakistan

autor

Ahmed Muhammad Masood

• Faculty of Pharmacy, Bahauddin Zakariya University Multan, Multan, Pakistan

autor

Abbas Nazar

• Department of Pharmaceutics, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Faisalabad, Pakistan

autor

Ashfaq Mehran

• Department of Pharmaceutics, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Faisalabad, Pakistan

autor

Iqbal Omeira

• Department of Pharmaceutics, Faculty of Pharmaceutical Sciences Government College University Faisalabad, Faisalabad, Pakistan

Bibliografia

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• 7. Sungthong, B.; Rattarom, R.; Sato, V. H.; Sato, H. Acta Chromatogr. 2019, 31, 241–5.
• 8.Abbas, G.; Hanif, M. Drug Dev. Ind. Pharm. 2018, 44, 2026–37.
• 9. Oliveira, K. R. W.; Sversut, R. A.; Singh, A. K.; Amaral, M. S.; Kassab, N. M. Acta Chromatogr. 2019, 31, 291–3.
• 10. Habibi, B.; Ghorbel-Abid, I.; Lahsini, R.; Ben Hassen, D. C.; Trabelsi-Ayadi, M. Acta Chromatogr. 2019, 31, 109–12.
• 11. Li, B.; Wang, Z.-W.; Lin, Q. B.; Hu, C. Y.; Su, Q. Z.; Wu, Y. M. J. Chromatogr. Sci. 2015, 53, 1026–35.
• 12. Koppala, S.; Ranga reddy, V.; Anireddy, J. S. J. Chromatogr. Sci. 2016, 54, 765–75.
• 13. Zaman, M.; Hanif, M.; Khan, N. U. H.; Mahmood, A.; Qaisar, M. N.; Ali, H. Acta Chromatogr. 2019, 31, 173–8.

Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).