Qualitative and quantitative analysis of seven signature components in the fruiting body of Antrodia cinnamomea by HPLC—ESI—MS/MS

• Autorzy: Huang H. S. , Hsu J. L. , Yu C. C. , Chang L. C. , Chen C. R. , Huang T. C. , Chang C. J.
• Języki publikacji: EN

Abstrakty

EN

An accurate and rapid liquid chromatography–electrospray ionizaion– tandem mass spectrometry (LC—ESI—MS/MS) analytical method was developed and validated for the simultaneous determination of antcins A, B, C, H, and K, dehydroeburicoic acid, and 4,7-dimethoxy-5-methyl-1,3-benzodioxole in the extract and capsule of Antrodia cinnamomea (AC) fruiting body. These seven signature compounds were ionized using an electrospray ion source and analyzed by a triple-quadrupole mass analyzer under a multiple reaction monitoring (MRM) mode. The MRM transitions of m/z 453/409 (antcin A), m/z 467/408 (antcin B), m/z 469/425 (antcin C), m/z 485/413 (antcin H), m/z 487/407 (antcin K), m/z 467/337 (dehydroeburicoic acid), and m/z 197/139 (4,7-dimethoxy-5-methyl-1,3-benzodioxole) were used to quantify these seven components, respectively. Their calibration curves presented good linear regressions (R2 > 0.997) within the tested concentration range. The intra- and inter-day precisions were less than 1.97% and 2.53%, respectively. The overall recovery was in the range of 87.55%–95.41%. This validated high-performance liquid chromatography (HPLC)—MS/MS method offers promising applications for the accurate and rapid quantification of signature compounds in the fruiting body and its commercial products.

Słowa kluczowe

EN

Antrodia cinnamomea; multiple reaction monitoring (MRM); tandem mass spectrometry; triterpenoid

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2016
• Tom: Vol. 28, no. 3
• Strony: 387--402
• Opis fizyczny: Bibliogr. 31 poz., rys., tab.

Twórcy

autor

Huang H. S.

• Department of Food Science, National Pingtung University of Science and Technology, Pintung 912, Taiwan

autor

Hsu J. L.

• esearch Center for Austronesian Medicine and Agriculture, National Pingtung University of Science and Technology, Pingtung 912 , Taiwan

autor

Yu C. C.

• Biotech Total Solutions Co., Ltd., New Taipei City 116, Taiwan

autor

Chang L. C.

• Biotech Total Solutions Co., Ltd., New Taipei City 116, Taiwan

autor

Chen C. R.

• Department of Life Science, National Taitung University, Taitung 950, Taiwan

autor

Huang T. C.

• Department of Food Science, National Pingtung University of Science and Technology, Pintung 912, Taiwan

autor

Chang C. J.

• Department of Biological Science and Technology, National Pingtung University of Science and Technology, Pingtung 912, Taiwan

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.