GC-MS analysis of volatile secondary metabolites in “Mediterranean” and “Continental” Festuca arundinacea (Poaceae) infected with the fungal endophyte Neotyphodium coenophialum strain AR542

• Autorzy: Qawasmeh A , Bourke C , Lee S. , Gray M , Wheatley W , Sucher N. J , Raman A
• Języki publikacji: EN

Abstrakty

EN

Profiles of volatile secondary metabolites (VSM) in Mediterranean and Continental Festuca arundinacea, either endophyte free or infected with the fungal endophyte Neotyphodium coenophialum strain AR542, were determined using gas chromatography-mass spectrometry (GC-MS). The profile of VSM in the endophyte-free Mediterranean F. arundinacea germplasm was similar to that of endophyte-free Continental F. arundinacea germplasm. However, the VSM profile in AR542-infected Mediterranean F. arundinacea was different to that in AR542-infected Continental F. arundinacea. Compound 1, identified as N-acetylnorloline, was detected in AR542-infected Mediterranean F. arundinacea as being sevenfold greater compared with its level in AR542-infected Continental F. arundinacea. Levels of compounds 2, 4, and 5 detected in AR542-infected Mediterranean F. arundinacea were significantly lower when compared with their levels in the AR542-infected Continental F. arundinacea. Levels of compound 3 were similar in both germplasms infected with endophyte strain AR542. The levels of compounds 2, 4, and 5 but not compound 3 were different between AR542 infected and endophyte free depending on germplasm. On the basis of the mass spectra obtained, compounds 2, 3, 4, and 5 were identified as tridecanoic acid methyl ester, n-capric acid, 11, 14, 17-eicosatrienoic acid, and linoleic acid ethyl ester, respectively. Our results highlight key differences between the Mediterranean and Continental germplasms. Comparison of the VSM of AR542-infected Mediterranean F. arundinacea with AR542-infected Continental F. arundinacea showed that there are quantitative differences between the two germplasms. These differences, which may impact on grazing systems involving horses, most probably arose as a result of intrinsic genetic differences between the two germplasms and are yet to be indentified.

Słowa kluczowe

EN

Festuca arundinacea; Neotyphodium coenophialum; endophyte; N-acetylnorloline

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 4
• Strony: 621--628
• Opis fizyczny: Bibliogr. 20 poz., rys., tab.

Twórcy

autor

Qawasmeh A

• Charles Sturt University Faculty of Science P.O. Box 883 Orange NSW 2800 Australia

autor

Bourke C

• Orange Agricultural Institute Department of Primary Industries Orange NSW 2800 Australia

autor

Lee S.

• University of Western Sydney Centre for Complementary Medicine Research B3, Bankstown Campus Locked Bag 1797 Penrith South DC NSW 1797 Australia

autor

Gray M

• University of Western Sydney Centre for Complementary Medicine Research B3, Bankstown Campus Locked Bag 1797 Penrith South DC NSW 1797 Australia

autor

Wheatley W

• Charles Sturt University Faculty of Science P.O. Box 883 Orange NSW 2800 Australia

autor

Sucher N. J

• University of Western Sydney Centre for Complementary Medicine Research B3, Bankstown Campus Locked Bag 1797 Penrith South DC NSW 1797 Australia

autor

Raman A

• Charles Sturt University Faculty of Science P.O. Box 883 Orange NSW 2800 Australia
• E H Graham Centre for Agricultural Innovation P.O. Box 883 Orange NSW 2800 Australia

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