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Partitioning of cerrena unicolor laccase activity in an aqueous two-phase system

Treść / Zawartość
Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Culture supernatant containing laccase produced by Cerrena unicolor strain was used to examine laccase partitioning between phases in an aqueous two-phase system. The investigated system consisted of polyethylene glycol 3000 and sodium phosphate buffer adjusted to pH = 7. Influence of several parameters on partitioning was measured, including phase forming components’ concentrations, tie line lengths, phase volume ratio, supernatant dilution, process temperature and halogen salt supplementation. Partitioning coefficients up to 78 in the bottom phase were achieved with yields of over 90%. Tie line length and phase volume ratio had significant effect on enzyme partitioning.
Słowa kluczowe
Rocznik
Strony
269--280
Opis fizyczny
Bibliogr. 25 poz., rys., tab.
Twórcy
  • Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź , Poland
autor
  • Dortmund University of Technology, The Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany
autor
  • Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź , Poland
  • Dortmund University of Technology, The Department of Biochemical and Chemical Engineering, Emil-Figge-Straße 70, D-44227 Dortmund, Germany
  • Lodz University of Technology, Faculty of Process and Environmental Engineering, ul. Wólczańska 213, 90-942, Łódź , Poland
Bibliografia
  • 1. Azevedo A.M., Rosa P.A.J., Ferreira I.F., Aires-Barros M.R., 2009. Chromatography-free recovery of biopharmaceuticals through aqueous two-phase processing. Trends Biotech., 27, 240-247. DOI: 10.1016/j.tibtech.2009.01.004.
  • 2. Benavides J., Rito-Palomares M., 2008. Practical experiences from the development of aqueous two-phase processes for the recovery of high value biological products. J. Chem. Technol. Biotechnol., 83, 133-142. DOI: 10.1002/jctb.1844.
  • 3. Bryjak J., Rekuć A., 2009. Effective purification of Cerrena unicolor laccase using microfiltration, ultrafiltration and acetone precipitation. App. Biochem. Biotechnol., 160, 2219-2235. DOI: 10.1007/s12010-009-8791-9.
  • 4. Claus H., Filip Z., 1997. The evidence for a laccase-like enzyme activity in a Bacillus sphaericus strain. Microbiol. Res., 152, 209–216. DOI: 10.1016/S0944-5013(97)80014-6.
  • 5. Diamantidis G., Effosse A., Potier P., Bally R., 2001. Purification and characterization of the first bacterial laccase in the rhizospheric bacterium Azospirillum lipoferum. Soil Biol. Biochem., 32, 919–927. DOI: 10.1016/S0038-0717(99)00221-7.
  • 6. Diamond A., Hsu J., 1989. Fundamental studies of biomolecule partitioning in aqueous two-phase systems. Biotech. Bioeng., 7, 1000-1014. DOI: 10.1002/bit.260340714. Enebak S.A., Blanchette R.A., 1989. Canker formation and decay in sugar maple and paper birch infected by Cerrena unicolor. Can. J. For. Res., 19, 225-231.
  • 7. Gu Z., Glatz C.E., 2007. Aqueous two-phase extraction for protein recovery from corn extracts. J. Chromatogr. B, 845, 38-50. DOI: 10.1016/j.jchromb.2006.07.025. Harvey B.M., Walker J.R.K., 1999. Studies with plant laccases: I. Comparison of plant and fungal laccases. Biochem. Mol. Biol. Biophys., 3, 45–51.
  • 8. Johansson G., Reczey K., 1998. Concentration and purification of b-glucosidase from Aspergillus niger by using aqueous two-phase partitioning. J. Chromatogr. B, 711, 161-172. DOI: 10.1016/S0378-4347(97)00601-4.
  • 9. Kim C.W., Rha C., 2000. Interfacial tension of polyethylene glycol/potassium phosphate aqueous two-phase systems. Phys. Chem. Liq., 38, 25-34. DOI: 10.1080/00319100008045294. Kula M.R., Kroner K.H., Hustedt H., 1982. Purification of enzymes by liquid-liquid extraction. Adv. Biochem. Eng., 50, 74-118. DOI: 10.1007/3-540-11699-0_11.
  • 10. Lindeberg G., Holm G., 1952. Occurrence of tyrosinase and laccase in fruit bodies of mycelia of some Hymenomycetes. Physiol. Plant, 5, 100-114. DOI: 10.1111/j.1399-3054.1952.tb08234.x.
  • 11. Liu Y., Wu Z., Zhang Y., Yuan H., 2012. Partitioning of biomolecules in aqueous two-phase systems of polyethylene glycol and nonionic surfactant. Biochem. Eng. J., 69, 93-99. DOI: 10.1016/j.bej.2012.08.018.
  • 12. Luterek J., Gianfreda L., Wojtaś-Wasilewska L., Cho M., Rogalski N.S., Jaszek J., Malarczyk M., Staszczak M., Fink-Boots M., Leonowicz A., 1998. Activity of free and immobilized extracellular Cerrena unicolor laccase in water miscible organic solvents. Holzforsch., 52 (6), 589-595.
  • 13. Majcherczyk A., Johannes Ch., Hüttermann A., 1998. Oxidation of polycyclic aromatic hydrocarbons (PAH) by laccase of trametes versicolor. Enzym. and Microb. Technol., 22, 335-341. DOI: 10.1016/S0141-0229(97)00199-3.
  • 14. Mayolo-Deloisa K., Trejo-Hernández M.R., Rito-Palomares M., 2009. Recovery of laccase from the residual compost of Agaricus bisporus in aqueous two-phase systems. Process Biochem., 44, 435-439. DOI: 10.1016/j.procbio.2008.12.010.
  • 15. Mehrnoush A., Mustafa S., Sarker M.I., Yazid A.M.M., 2012. Optimization of serine protease purification from mango (Mangifera Indica cv. Chokanan) peel in polyethylene glycol/dextran aqueous two phase system. Int. J. Mol. Sci., 13, 3636-3649. DOI: 10.3390/ijms13033636. Pazuki G.R., Taghikhani V., Vossoughi M., 2009. modeling process partitioning of biomolecules in polymerpolymer and polymer-salt aqueous two-phase systems (ATPS) using and extended excess Gibbs energy model. Z. Phys. Chem., 223, 263-278. DOI: 10.1524/zpch.2009.5421. Prinz A., Koch K., Górak A., Zeiner T., 2014. Multi-stage laccase extraction and separation using aqueous two-phase systems: Experiment and model. Process Biochem., 49, 1020-1031. DOI: 10.1016/j.procbio.2014.03.011.
  • 16. Prinz A., Zeiner T., Vössing T., Schüttmann I., Zorn H., Górak A., 2012. Experimental investigation of laccase purification using aqueous two-phase extraction. Chem. Eng. Trans., 27, 349-354.
  • 17. Rajeeva S., Lele. S.S., 2010. Bioprocessing of laccase produced by submerged culture of Ganoderma sp. WR-1. Sep. Purif. Technol., 76, 110-119. DOI: 10.1016/j.seppur.2010.09.027.
  • 18. Ratanapongleka K., Phetsom J., 2011. extraction in two-phase systems and some properties of laccase from Lentinus polychrous. Eng. Technol., 57, 812-815.
  • 19. Reschke T., Brandenbusch C., Sadowski G., 2014. Modeling aqueous two-phase systems: I. Polyethylene glycol and inorganic salts as ATPS former. Fluid Phase Equilib., 368, 91-103. DOI: 10.1016/j.fluid.2014.02.016.
  • 20. Rogalski J., Dawidowicz A., Jóźwik. E., Leonowicz A., 1999. Immobilization of laccase from Cerrena unicolor on controlled porosity glass. J. Mol. Catal. B: Enzym., 6, 29–39. DOI: 10.1016/S1381-1177(98)00117-9.
  • 21. Salabat A., Moghadam S.T., Far M.R., 2010. Liquid-liquid equilibria of aqueous two-phase systems composed of TritonX-100 and sodium citrate or magnesium sulfate salts. Calphad, 34, 81-83. DOI: 10.1016/j.calphad.2009.12.004.
  • 22. Silvério S.C., Rodríguez O., Tavares A.P.M., Teixeira J.A., Macedo E.A., 2012. Laccase recovery with aqueous two-phase systems: Enzyme partitioning and stability. J. Mol. Catal. B: Enzym., 87, 37-43. DOI: 10.1016/j.molcatb.2012.10.010.
  • 23. Yang Y., Ding Y., Liao X., Cai Y., 2013. Purification and characterization of a new laccase from Shiraia sp.SUPER-H168. Process Biochem., 48, 351-357. DOI: 10.1016/j.procbio.2012.12.011.
  • 24. Xu F., 1996. Oxidation of phenols, anilines, and benzenethiols by fungal laccases: correlation between activity and redox potentials as well as halide inhibition. Biochem., 35, 7608–7614. DOI: 10.1021/bi952971a.
  • 25. Yoshida H., 1883. Chemistry of lacquer (Urushi). J. Chem. Soc., 43, 472–48.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-f076dc88-9648-4a0c-85b7-dea40e8802e5
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