Study on the separation of triterpene saponins from Panax notoginseng by off-line two-dimensional supercritical fluid chromatography–ultrahigh-performance liquid chromatography

Xing, Q.; Fu, Q.; Liang, X.

doi:10.1556/1326.2017.28405

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Tytuł artykułu

Study on the separation of triterpene saponins from Panax notoginseng by off-line two-dimensional supercritical fluid chromatography–ultrahigh-performance liquid chromatography

Autorzy

Xing Q. , Fu Q. , Liang X.

Wybrane pełne teksty z tego czasopisma

Identyfikatory

DOI

10.1556/1326.2017.28405

Warianty tytułu

Języki publikacji

Abstrakty

The off-line two-dimensional supercritical fluid chromatography (SFC)–ultrahigh-performance liquid chromatography (UHPLC) was selected to separate the triterpene saponins from Panax notoginseng. The separation by SFC was performed on an Atlantis® HILIC silica column. Methanol was selected as a modifier, and the most time-saving gradient was developed. The decrease of the column temperature and the increase of the back pressure could shorten the retention time but had no effect on the separation selectivity. Then, the back pressure, column temperature, and flow rate were set as 131 bar, 45 °C, and 4.0 mL min−1, respectively. The retention behavior of the saponins from P. notoginseng was different between SFC and reversed-phase liquid chromatography (RPLC), which facilitated to construct an off-line SFC/RPLC–mass spectrometry (MS) system. In first dimension, a total of eight fractions were collected under SFC and further analyzed by RPLC–MS in second dimension. The result indicated that the retention behavior of triterpene saponins was mainly controlled by the hydrogen bonding interactions which were affected by the number and types of sugars, as well as the aglycone in the structure of triterpene saponins. Thus, the presence of “clustering effect” under SFC was observed, namely, one SFC peak always contained several saponins with same number of sugars and similar structure of aglycone. The clustering effect of triterpene saponins promised SFC to be used as first dimension to complete the preliminary crude separation in the two-dimensional liquid chromatography.

Słowa kluczowe

Supercritical fluid chromatography reversed-phase liquid chromatography mass spectrometry Panax notoginseng saponins

Wydawca

University of Silesia in Katowice
Akademiai Kiado

Czasopismo

Acta Chromatographica

Rocznik

2017

Tom

Vol. 29, no. 4

Strony

497--502

Opis fizyczny

Bibliogr. 37 poz., rys., tab.

Twórcy

autor

Xing Q.

Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

autor

Fu Q.

Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

autor

Liang X.

Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, School of Pharmacy, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

Bibliografia

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).

Typ dokumentu

Bibliografia

Identyfikator YADDA

bwmeta1.element.baztech-f06c6bf0-3d29-4770-bb50-2df1b5480b29