In vitro cytotoxicity of biodegradable Zn-Mg surgical wires in tumor and healthy cells

• Autorzy: Milenin Andrij , Łukowicz Krzysztof , Truchan Karolina , Osyczka Anna Maria

Identyfikatory

DOI

10.37190/ABB-01927-2021-01

• Języki publikacji: EN

Abstrakty

EN

In this work, we examined the in vitro cytotoxicity of new biodegradable surgical wires. The wires made of zinc with the addition of a small amount of magnesium (pure zinc, ZnMg 0.0026, ZnMg 0.0068, and ZnMg 0.08) have been investigated. The wires were produced using a technology based on extrusion and subsequent drawing. The resulting wires with a diameter of 0.8–1.0 mm are designed to be used in surgical operations related to bone joints. For cytotoxicity studies, we have selected human dental pulp stem cells (hDPSC) as the cell population representing normal osteoprogenitor cells. Considering that, after bone surgeries, the chance of osteosarcoma increases, we have compared the results obtained in hDPSC to those obtained with Saos-2 human osteosarcoma cell line. Cultured cells were exposed to the extracts obtained from the materials incubated in culture medium for 24 h with and without preincubation. Extracts of different ratios were examined. The results showed that the extracts obtained from wires made of ZnMg 0.0026 alloy exhibit high toxicity to Saos-2 osteosarcoma cells and low toxicity to hDPSC cells. This was in contrast to all reference materials, i.e., commercial surgical sutures made of steel and polymers, that did not display cytotoxicity toward osteosarcoma cells. Thus, the detected phenomenon for the ZnMg 0.0026 alloy can become the basis for creating biodegradable Zn-Mg surgical wires with antitumor activity.

Słowa kluczowe

EN

surgical wire; ZnMg alloys; tumor; drawing; biodegradation; cytotoxicity

PL

drut chirurgiczny; stopy ZnMg; nowotwór; ciągnienie; biodegradacja; cytotoksyczność

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2021
• Tom: Vol. 23, no. 4
• Strony: 85--93
• Opis fizyczny: Bibliogr. 23 poz., rys.

Twórcy

autor

Milenin Andrij

• AGH University of Science and Technology, Faculty of Metals Engineering and Industrial Computer Science

autor

Łukowicz Krzysztof

• Jagiellonian University, Faculty of Biology, Institute of Zoology and Biomedical Research, Department of Cell Biology and Imaging

autor

Truchan Karolina

• Jagiellonian University, Faculty of Biology, Institute of Zoology and Biomedical Research, Department of Cell Biology and Imaging

autor

Osyczka Anna Maria

• Jagiellonian University, Faculty of Biology, Institute of Zoology and Biomedical Research, Department of Cell Biology and Imaging

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2024).