A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

• Autorzy: Çelebi B.

Identyfikatory

DOI

10.1556/1326.2017.29.2.00

• Języki publikacji: EN

Abstrakty

EN

New polyacrylate-based monosized-porous polymer beads were proposed as a stationary phase for the separation of polar compounds by microbore reversed-phase chromatography. For this purpose, monosized-porous poly(glycerol dimethacrylate-co-glycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA), beads with hydroxyl functionality were synthesized by a modified seeded polymerization. The selected octadecylating agent, stearoyl chloride (SC), was covalently attached onto the hydrophilic beads via a direct, single stage reaction with a simple synthetic route. SC attached-poly( GDMA-co-GDGDA) beads were slurry-packed into the microbore columns and used as separation medium microbore reversed-phase chromatography. The stationary phase was used for separation of alkylbenzenes and polar analytes by micro reversed-phase chromatography, using mobile phases with low acetonitrile content. Theoretical plate number (TPN) values up to 12,000 plates m−1 and 10,000 plates m−1 for alkylbenzenes and polar analytes, respectively, were achieved. The results also showed that poly(GDMA-co-GDGDA) hydrogel beads are a promising starting material for a number of chromatographic applications like reversed-phase (RP) chromatography, hydrophilic interaction chromatography (HILIC), ion-exchange chromatography (IEC), and affinity chromatography with a single-stage surface modification.

Słowa kluczowe

EN

reversed-phase chromatography; stearoyl chloride; hydrogel beads; microbore columns; polar analytes

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2017
• Tom: Vol. 29, no. 2
• Strony: 143--159
• Opis fizyczny: Bibliogr. 33 poz., rys., tab.

Twórcy

autor

Çelebi B.

• Hacettepe University, Chemical Engineering Department, Ankara, Turkey

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).