Development and validation of HPTLC-densitometric method for estimation of trigonelline in leaves of Abrus precatorius L. and its formulation

• Autorzy: Kumbhalkar B. B , Rajopadhye A. A , Upadhye A. S
• Języki publikacji: EN

Abstrakty

EN

A new, simple and reproducible HPTLC-densitometric method has been established and validated for estimation of trigonelline in leaves of Abrus precatorius L. and its herbal formulation Chatak® using ICH guidelines. Accelerated solvent extraction (ASE) as an alternative to convention techniques was also explored for the rapid extraction. The methanol extracts of leaves, its formulation, and standard solution of trigonelline were applied on silica gel F254 HPTLC plates and developed in twin chamber using mobile phase toluene-ethyl acetate-formic acid-methanol (2:3:1.8:3.8, v/v/v/v). The plates were scanned at 268 nm (λmax of trigonelline) using Camag TLC scanner 3 with CATS 4 software. A linear relationship was obtained between response (peak area) and amount of trigonelline in the range 40–200 ng spot−1; the correlation coefficient was 0.9957. ASE method has higher extraction efficiency in less time as compared to Soxhlet extraction. The HPTLC-densitometric method showed good linearity, recovery and high precision of compound. It is useful to analyze trigonelline in A. precatorius leaves and routine quality control of its marketed formulation.

Słowa kluczowe

EN

trigonelline; Abrus precatorius L; accelerated solvent extraction; HPTLC-densitometry; ICH guidelines

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 3
• Strony: 511--518
• Opis fizyczny: Bibliogr. 10 poz., rys., tab.

Twórcy

autor

Kumbhalkar B. B

• Agharkar Research Institute Botany, Plant Science Division G. G. Agarkar Road Pune 411 004 India

autor

Rajopadhye A. A

• Agharkar Research Institute Botany, Plant Science Division G. G. Agarkar Road Pune 411 004 India

autor

Upadhye A. S

• Agharkar Research Institute Botany, Plant Science Division G. G. Agarkar Road Pune 411 004 India

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