Simultaneous determination of six triterpenoid saponins in beagle dog plasma by UPLC-MS/MS and its application to a pharmacokinetic study after oral administration of the extract of the Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves

• Autorzy: Wang Zhibin , Chang Yaodan , Cao Feng , Yang Chunjuan , Wang Zhenyue , Kuang Haixue

Identyfikatory

DOI

10.1556/1326.2022.01011

• Języki publikacji: EN

Abstrakty

EN

A rapid and simple ultra-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed and validated for simultaneous determination of six analytes from the Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. leaves (ESL) in beagle dog plasma for the first time, including 3-O-α-ʟ-rhamnopyranosyl-(1→2)-α-ʟ-arabinopyranoside-29-hydroxy oleanolic acid, 3-O-β-ᴅ-glucopyranosyl-(1→2)-α-ʟ-arabinopyranoside-29-hydroxy oleanolic acid, 3-O-β-ᴅ-glucopyranosyl-(1→2)-α-ʟ-arabinopyranosyl-30-norlean-12,20 (29) –dien-28-olic acid, ciwujianoside E, guaianin N, and eleutheroside K. The chromatographic separation was performed using an ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) using a gradient elution way with a mobile phase of acetonitrile-water containing 0.1% formic acid. Analytes were detected on a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source with multiple reaction monitoring (MRM) mode. Calibration curves were all linear (r ≥ 0.9933) over the concentration range. The mean extraction recoveries and matrix effect of analytes and I.S. were ranged from 80.26% to 98.32% and from 91.27% to 111.67%, respectively. The intra-day and inter-day precision were ranged from 2.20% to 14.81%, and the accuracy range was 1.60–14.60%. The analytical method was successfully applied for the pharmacokinetic characteristics of the six analytes in beagle plasma after oral administration of ESL extracts. The T_1/2 of six analytes was more than 3.09 ± 0.78 h.

Słowa kluczowe

EN

Eleutherococcus senticosus (Rupr. & Maxim.) Maxim.; Pharmacokinetics; Triterpenoid saponins; UPLC-MS/MS

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2023
• Tom: Vol. 35, no. 1
• Strony: 88--98
• Opis fizyczny: Bibliogr. 30 poz., tab., rys., wykr.

Twórcy

autor

Wang Zhibin

• Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Xiangfang District, Harbin 150040, China

autor

Chang Yaodan

• Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Xiangfang District, Harbin 150040, China

autor

Cao Feng

• Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Xiangfang District, Harbin 150040, China

autor

Yang Chunjuan

• Harbin Medical University, College of Pharmacy, 157 Baojian Road, Nangang District, Harbin 150040, China

autor

Wang Zhenyue

• Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Xiangfang District, Harbin 150040, China

• https://orcid.org/0000-0002-4268-3749

autor

Kuang Haixue

• Key Laboratory of Basic and Application Research of Beiyao (Heilongjiang University of Chinese Medicine), Ministry of Education, 24 Heping Road, Xiangfang District, Harbin 150040, China

• https://orcid.org/0000-0002-5760-007X

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).