Nmr studies of human cystatin C - stable isotope labeling of human cystatin C

• Autorzy: Jurczak P. , Maszota-Zieleniak M.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to obtain a double and triple labeled hu man cystatin C (h CC ). Another objective was to record sets of 2D and 3D NMR spectra of the h CC dimer (in a solution) using a 700MHz spectrometer. The data obtained durin g attempts to determine the NMR structure should provide useful information about chemical shifts of amino acid residues. They will certainly accelerate solving the human cys tatin C NMR structure. In this paper the main focus is put on triple isotopic labeling, protein overproduction and NMR analysis of h CC . The first two processes lead to obtaining h CC labeled with stable isotopes of carbon ( 13 C), nitrogen ( 15 N) and hydrogen ( 2 H) (double labeled h CC was obtained with a similar method). The obtained protein was later used for the purp ose of NMR spectra.

Słowa kluczowe

EN

NMR; overproduction; stable isotopes; proteins; labeling

PL

NMR; nadprodukcja; stabilne izotopy; białka; etykietowanie

• Wydawca: Politechnika Gdańska
• Czasopismo: TASK Quarterly : scientific bulletin of Academic Computer Centre in Gdansk
• Rocznik: 2014
• Tom: Vol. 18, No 4
• Strony: 327--330
• Opis fizyczny: Bibliogr. 7 poz., rys.

Twórcy

autor

Jurczak P.

• Faculty of Chemistry, University of Gdansk Wita Stwosza 63, 80-308 Gdansk, Poland

autor

Maszota-Zieleniak M.

• Faculty of Chemistry, University of Gdansk Wita Stwosza 63, 80-308 Gdansk, Poland

Bibliografia

• [1] Levy E, Jaskólski M, Grubb A 2006 Brain Pathol. 16 (1) 60
• [2] Mussap M, Plebani M 2004 Critical Rev. in Clinical Lab. Sciences 41 (5–6) 467
• [3] Janowski R, Kozak M, Abrahamson M, Grubb A 2005 Proteins 61(3) 570
• [4] Liu Y, Eisenberg D 2002 Protein Sci. 11 (6) 1285
• [5] Ekiel I, Abrahamson M, Fulton D B, Lindahl P, Storer A C, Levadoux W, Lafrance M, Labelle S, Pomerleau Y, Groleau D, LeSauteur L 1997 J. Mol. Biol. 271 (2) 266
• [6] Keller R 2004 The Computer Aided Resonance Assignment Tutorial , ISBN 3-85600-112-3, CANTINA Verlag
• [7] Wishart D S, Arndt D, Berjanskii M, Tang P, Zhou J, Lin G 2008 Nucleic Acids Res 36 496