Synthesis of isoamyl acetate using protein-coated microcrystals of different lipases

• Autorzy: Türk Murat

Identyfikatory

DOI

10.2478/pjct-2023-0012

• Języki publikacji: EN

Abstrakty

EN

The goal of this study was the immobilization of different lipases as protein-coated microcrystals on K₂SO₄ and their uses in the synthesis of isoamyl acetate in n-hexane medium. The optimum conditions, such as lipase variety, temperature, the initial molar ratio of vinyl acetate/isoamyl alcohol, immobilized lipase amount, and reaction time were determined. The highest conversion was obtained when protein-coated microcrystals of Thermomyces lanuginosus lipase (TLL-PCMCs) was used for the synthesis of isoamyl acetate. The optimum temperature, the initial molar ratio of vinyl acetate/isoamyl alcohol, immobilized lipase amount, and reaction time were determined to be 50 °C, 3.0, 30 mg, and 360 min, respectively. Under the optimized conditions, isoamyl acetate yield was obtained as 95%. TLL-PCMCs retained 90% of their initial activity after five repeat use in the isoamyl acetate synthesis. TLL-PCMCs may be used in the preparation of industrially important aroma compounds due its ease of preparation and efficiency.

Słowa kluczowe

EN

lipase; immobilization; protein-coated microcrystals; isoamyl acetate

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2023
• Tom: Vol. 25, nr 2
• Strony: 15--20
• Opis fizyczny: Bibliogr. 47 poz., rys., tab., wz.

Twórcy

autor

Türk Murat

• Cukurova University, Ceyhan Vocational School, 01330, Adana, Turkey

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).