The interaction of laser radiation with tissue in the aspect of generating the process of decellularization in the preparation of animal origin autologous tissue

• Autorzy: Kopernik Magdalena , Major Roman , Lis Grzegorz , Wilczek Piotr , Lis Maciej , Imbir Gabriela , Chrouda Abir , Mzyk Aldona , Ostrowski Roman , Sanak Marek

Identyfikatory

DOI

10.37190/ABB-01519-2019-03

• Języki publikacji: EN

Abstrakty

EN

Purpose: The aim of the work was to create an appropriate substrate for organ transplantation using bioactive tissue-based scaffold populated by cells of the graft recipient. The purpose of the modeling was to investigate the mechanical effects of wave loading of aortic and pulmonary tissue material. Methods: The biological properties of tissues of aortic and pulmonary valves were modified by the process of decellularization. The host cells were removed by various physical methods with focus on minimal degradation of the extracellular matrix. Thus, the decellularization process was controlled by histological methods. The tissue decellularization process was simulated by finite element modelling. Results: The mechanical results represented by a displacement at the center of the sample were coherent and the heterogeneity of the distribution of the caves on the surface of the samples was confirmed, both by experiment and in the simulation by the alternate occurrence of local minima and maxima. The latter results from the uneven removal of cells from the effect of the wave causing decellularization were also predicted by the numerical model. Laser radiation had a destructive effect on the components of the extracellular matrix (e.g., collagen and elastic fibers), mainly depending on the fluence and number of pulses in a single exposure. Conclusions: The differences between the valve tissue materials were shown, and the impact of the process of decellularization on the properties of the tissues was analyzed. It should be emphasized that due to low absorption and high scattering, laser radiation can deeply penetrate the tissue, which allows for effective decellularization process in the entire volume of irradiated tissue.

Słowa kluczowe

EN

decellularized tissue; aortic valve; finite element modeling; histology; pulmonary valve; laser radiation

PL

zastawka aorty; FEM; histologia; promieniowanie laserowe

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2020
• Tom: Vol. 22, no. 1
• Strony: 67--77
• Opis fizyczny: Bibliogr. 22 poz., rys., tab., wykr.

Twórcy

autor

Kopernik Magdalena

• AGH University of Science and Technology, Kraków, Poland

autor

Major Roman

• Institute of Metallurgy and Materials Science, Polish Academy of Sciences, Kraków, Poland

autor

Lis Grzegorz

• Jagiellonian University Medical College, Department of Histology, Kraków, Poland

autor

Wilczek Piotr

• Foundation for Cardiac Surgery Development, Bioengineering Laboratory, Zabrze, Poland
• The President Stanislaw Wojciechowski State University of Applied Sciences in Kalisz, Faculty of Health Sciences, Kalisz, Poland

autor

Lis Maciej

• Jagiellonian University Medical College, Department of Histology, Kraków, Poland

autor

Imbir Gabriela

• Institute of Metallurgy and Materials Science, Polish Academy of Sciences, Kraków, Poland

autor

Chrouda Abir

• Institute of Metallurgy and Materials Science, Polish Academy of Sciences, Kraków, Poland

autor

Mzyk Aldona

• Institute of Metallurgy and Materials Science, Polish Academy of Sciences, Kraków, Poland

autor

Ostrowski Roman

• Military University of Technology, Institute of Optoelectronics, Warsaw, Poland

autor

Sanak Marek

• Jagiellonian University Medical College, Department of Medicine, Kraków, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).