Assessment of cytotoxic and antimicrobial activity of selected gingival haemostatic agents – in vitro study

• Autorzy: Szymonowicz Maria , Rusak Agnieszka , Pajączkowska Magdalena , Nowicka Joanna , Wiśniewska Kamila , Żywicka Bogusława , Żywicka Bogusława , Rybak Zbigniew , Dobrzyński Maciej

Identyfikatory

DOI

10.37190/ABB-01649-2020-03

• Języki publikacji: EN

Abstrakty

EN

Purpose: The present research aimed to determine whether and how the aluminium chloride – based materials affect the cell line of the bacterial line and fungi. Methods: Cytotoxicity of haemostatic astringents: Alustat (liquid), Alustat (gel), Alustat (foam), Alustin, Hemostat, Racestyptine and Traxodent containing AlCl3 was conducted on L929 cell line with the use of MTT and SRB assays. The antimicrobial activity (CFU and MIC) against C. albicans, S. mutans, L. rhamnosus was determined. Results: In the MTT results, cell viability for all agents were very low. In SRB, the lowest cytotoxicity was demonstrated for Hemostat and Alustat (foam), Traxodent and Racestyptine. Total reduction of the CFU of S. mutans was observed. Alustat (gel) and Alustat (liquid) completely inhibited the growth of C. albicans, S. mutans and L. rhamnosus. Conclusions: The viability of L929 cells obtained in the SRB assay is more reliable than that obtained in the MTT assay, in the case of gingival haemostatic agents.

Słowa kluczowe

EN

haemostatic agents; cytotoxicity; gingiva retraction; antimicrobial activity

PL

środki hemostatyczne; cytotoksyczność; retrakcja dziąsła; Aktywność przeciwbakteryjna

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2020
• Tom: Vol. 22, no. 3
• Strony: 185--198
• Opis fizyczny: Bibliogr. 25 poz., il., rys., tab., wykr.

Twórcy

autor

Szymonowicz Maria

• Department of Experimental Surgery and Biomaterials Research, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland

autor

Rusak Agnieszka

• Department of Human Morphology and Embryology, Division of Histology and Embryology, Wroclaw Medical University, ul. T. Chałubińskiego 6a, 50-368 Wrocław, Poland

autor

Pajączkowska Magdalena

• Department and Division of Microbiology, Faculty of Medicine, Wroclaw Medical University, Wrocław, Poland

autor

Nowicka Joanna

• Department and Division of Microbiology, Faculty of Medicine, Wroclaw Medical University, Wrocław, Poland

autor

Wiśniewska Kamila

• Department of Experimental Surgery and Biomaterials Research, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland

autor

Żywicka Bogusława

• Department of Experimental Surgery and Biomaterials Research, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland

autor

Żywicka Bogusława

• Department of Experimental Surgery and Biomaterials Research, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland

autor

Rybak Zbigniew

• Department of Experimental Surgery and Biomaterials Research, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland

autor

Dobrzyński Maciej

• Department of Conservative Dentistry and Pedodontics, Faculty of Dentistry, Wroclaw Medical University, Wrocław, Poland

Bibliografia

• [1] AL HAMAD K.Q., AZAR W.Z., ALWAELI H.A., SAID K.N., A clinical study on the effects of cordless and conventional retraction techniques on the gingival and periodontal health, J. Clin. Periodontol., 2008, 35, 105, 3–8.
• [2] BALOUIRI M., SADIKI M., IBNSOUDA S.K., Methods for in vitro evaluating antimicrobial activity: A review, J. Pharm. Anal., 2016, 6, 71–9.
• [3] DE OLIVEIRA BERNADES K., HILGERT L.A., RIBEIRO A.P.D., GARCIA F.C.P., PEREIRA P.N.R., The influence of hemostatic agents on dentin and enamel surfaces and dental bonding: A systematic review, J. Am. Dent. Assoc., 2014, 145, 1120–1127.
• [4] DOBRZYŃSKI M., PAJĄCZKOWSKA M., NOWICKA J., JAWORSKI A., KOSIOR P., SZYMONOWICZ M. et al., Study of Surface Structure Changes for Selected Ceramics Used in the CAD/CAM System on the Degree of Microbial Colonization, in Vitro Tests, Biomed. Res. Int., 2019, 1–13.
• [5] HANSEN P.A., TIRA D.E., BARLOW J., Current methods of finish-line exposure by practicing prosthodontists, J. Prosthodont., 1999, 8, 163–70.
• [6] HSU B., LEE S., SCHWASS D., TOMPKINS G., Antimicrobial activity of chemomechanical gingival retraction products, J. Am. Dent. Assoc., 2017, 148, 493–499.
• [7] JAROSZEWSKA-WÓJCICKA A., NOWAKOWSKA D., Cytotoksyczność materiałów stosowanych w protetyce stomatologicznej – przegląd piśmiennictwa. Cytotoxicity of dental materials used in prosthodontics. A literature review*, Protet. Stomatol., 2016, 66, 295–306 (in Polish).
• [8] JO H.Y., KIM Y., PARK H.W., MOON H.E., BAE S., KIM J. et al., The Unreliability of MTT Assay in the Cytotoxic Test of Primary Cultured Glioblastoma Cells, Exp. Neurobiol., 2015, 24, 235–245.
• [9] KOPAC I., Viability of fibroblasts in cell culture after treatment with different chemical retraction agents, 1993, 98–104.
• [10] KOPAC I., STERLE M., MARION L., Electron microscopic analysis of the effects of chemical retraction agents on cultured rat keratinocytes, J. Prosthet. Dent., 2002, 87 (1), 51–56.
• [11] MOSMANN T., Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, J. Immunol. Methods, 1983, 65, 55–63.
• [12] MORAES MELO NETO C.L., BORGES JR H.F., FIRMINO DE SOUZA Y., SANTIN G.C., SABIO S., Comparison between aluminum chloride and tetryzoline hydrochloride for control of vertical gingival displacement and crevicular fluid, Rev. Odontol. UNESP, 2017, 46 (4), 220–226.
• [13] NOWAKOWSKA D., SACZKO J., KULBACKA J., CHOROMAŃSKA A., Dynamic oxidoreductive potential of astringent retraction agents, Folia Biol. (Praha), 2010, 56, 263–268.
• [14] NOWAKOWSKA D., SACZKO J., KULBACKA J., CHOROMAŃSKA A., RASZEWSKI Z., Cytotoxic potential of vasoconstrictor experimental gingival retraction agents – in vitro study on primary human gingival fibroblasts, Folia Biol. (Czech Republic), 2012, 58, 37–43.
• [15] PLUMB J.A., MILROY R., KAYE S.B., Effects of the pH Dependence of 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide-Formazan Absorption on Chemosensitivity Determined by a Novel Tetrazolium-based Assay, Cancer. Res., 1989, 49, 4435–4440.
• [16] SAFARI S., VOSSOGHI SHESHKALANI M., VOSSOGHI SHESHKALANI M., HOSEINI GHAVAM F., HAMEDI M., Gingival Retraction Methods for Fabrication of Fixed Partial Denture: Literature Review, J. Dent. Biomater., 2016, 3, 205–213.
• [17] SHI Q., MAAS L., VEITH C., VAN SCHOOTEN F.J., GODSCHALK R.W., Acidic cellular microenvironment modifies carcinogen-induced DNA damage and repair, Arch. Toxicol., 2017, 91, 2425–2441.
• [18] SKEHAN P., STORENG R., SCUDIERO D., MONKS A., MCMAHON J., VISTICA D. et al., New colorimetric cytotoxicity assay for anticancer-drug screening, J. Natl. Cancer Inst., 1990, 82, 1107–1112.
• [19] SZYMONOWICZ M., RYBAK Z., FRACZEK-SZCZYPTA A., PALUCH D., RUSAK A., NOWICKA K. et al., Haemocompatibility and cytotoxic studies of non-metallic composite materials modified with magnetic nano and microparticles, Acta Bioeng. Biomech., 2015, 17, 49–58.
• [20] TARIGHI P., KHOROUSHI M., A review on common chemical hemostatic agents in restorative dentistry, Dent. Res. J. (Isfahan), 2014, 11, 423–428.
• [21] VAN TONDER A., JOUBERT A.M., CROMARTY A.D., Limitations of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay when compared to three commonly used cell enumeration assays, BMC Res. Notes, 2015, 8, 1–10.
• [22] VON ARX T., JENSEN S.S., HÄNNI S., SCHENK R.K., Haemostatic agents used in periradicular surgery: An experimental study of their efficacy and tissue reactions, Int. Endod. J., 2006, 39, 800–808.
• [23] WANG X.-D., DENG R.-C., LIU Y., LI B., HO S.-H., XIAO Z.-P., Modification of MTT Assay for Precision and Repeatability and Its Mechanistic Implication, Asian J. Chem., 2014, 26, 8015–8018.
• [24] WOJDA S., SAJEWICZ E., Influence of dental materials on hardness and Young’s modulus of the surface layers of tooth enamel formed as a result of friction, Acta Bioeng. Biomech., 2019, 21 (1), 129–139.
• [25] YALÇIN M., BARUTCIGIL Ç., UMAR I., BOZKURT B.S., HAKKI S.S., Cytotoxicity of hemostatic agents on the human gingival fibroblast, Eur. Rev. Med. Pharmacol. Sci., 2013, 17, 984–988.