Quantitative analysis of marker constituent swertisin in Enicostemma hyssopifolium verdoon by RP-HPLC and HPTLC

• Autorzy: Patel M. B , Mishra S. H.
• Języki publikacji: EN

Abstrakty

EN

Methods based on RP-HPLC and HPTLC with UV detection for rapid quantitative determination of marker component in Enicostemma hyssopifolium, swertisin are described. The recovery of the compound was between 96.3–101.5% by HPTLC method and 98.9–100.2% by HPLC assay. The relative standard deviations ranged between 1.53–1.81 (intra-day) and 1.33–1.96 (inter-day) for HPTLC and 0.60–1.11 (intra-day) and 0.80–1.20 (inter-day) for HPLC. The methods were used for routine analysis of swertisin in the aerial parts of the plant. HPTLC method was found to be more economic and less time consuming than HPLC. Reproducibility and recovery in HPLC method were better than that in HPTLC method.

Słowa kluczowe

EN

Enicostemma hyssopifolium; swertisin; HPTLC; RP-HPLC

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 1
• Strony: 85--95
• Opis fizyczny: Bibliogr. 25 poz., rys., tab.

Twórcy

autor

Patel M. B

• Atmiya Institute of Pharmacy Department of Pharmacognosy and Phytochemistry Yogidham Gurukul, Kalawad Road Rajkot Gujarat India

autor

Mishra S. H.

• The M.S. University of Baroda Pharmacy Department, Faculty of Technology and Engineering, Kalabhavan Vadodara 390 001 Gujarat India

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