Characterization of oligoglucan-containing products derived from Mesorhizobium loti HAMBI 1148 murein after lysozyme digestion and β-elimination

• Autorzy: Karaś M , Russa R
• Języki publikacji: EN

Abstrakty

EN

Short glucan oligomers were found to be covalently linked to the peptidoglycan fragments liberated by partial lysozyme digestion of Mesorhizobium loti HAMBI 1148 murein and among some muropeptide mono and oligomers subsequently subjected to alkaline β-elimination. The mass spectra of the preparation before and after treatment with alkali contained deprotonated ions [M-H] − recorded at m / z 3107 and 2856 which represented muropeptide fragments with 2 or 3 glucose residues, respectively, at the reducing end. Because of the lack of a free reducing group, muramyl residues remained untouched during the β-elimination reaction in a manner comparable with muropeptide fragments protected by 1,6-anhydromuramic acid represented in the spectra by [M-H] − ions with masses of 2451 and 2762 m / z . The ion [M-H] − at m / z 1615 which was proposed to represent a disaccharide fragment with one Glc residue linked had no counterpart in the spectra of lysozyme-liberated muropeptides and was supposed to have arisen from a dimer (ions [M + H] + 2023 m / z and [M-H] − 2021 m / z observed for muropeptides not treated with alkali) after elimination of a GlcNAc-acetamidohexenopyranose fragment in the alkaline environment. According to a similar mechanism, the ion at m / z 2723 [M-H] − determined as a hexasaccharide fragment with an anhydromuramic acid residue at the end arose from a dimer represented by ion [M-H] − with mass 3128 m / z . The data presented suggest that glycan strands in rhizobium murein can be protected at the reducing end by short oligoglucans except for 1,6-anhydromuramic acid residues.

Słowa kluczowe

EN

peptidoglycan; rhizobium; glucan oligomer; β-elimination

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2010
• Tom: Vol. 22, no. 2
• Strony: 323--335
• Opis fizyczny: Bibliogr. 49 poz., rys., tab.

Twórcy

autor

Karaś M

• Maria Curie-Skłodowska University Department of Genetics and Microbiology 20-033 Lublin Poland

autor

Russa R

• Maria Curie-Skłodowska University Department of Genetics and Microbiology 20-033 Lublin Poland

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