Purification and recovery of laccase produced by submerged cultures of Trametes versicolor by three-phase partitioning as a simple and highly efficient technique

• Autorzy: Wasak A. , Drozd R. , Grygorcewicz B. , Jankowiak D. , Rakoczy R.

Identyfikatory

DOI

10.2478/pjct-2018-0059

• Języki publikacji: EN

Abstrakty

EN

In this work, three-phase partitioning (TPP) was used for the purification of laccase from liquid cultures of Trametes versicolor. For determining the optimal conditions of TPP process, parameters such as initial pH (6.5, 7.0, 7.5, 8.0), ammonium sulphate saturation (20%-80%) and the water phase to tert-butanol ratio (1:0.5, 1:1, 1:2), were analyzed. The best conditions with 73% recovery and 24-fold purification was obtained with the use of 50% saturation with ammonium sulphate, water phase to tert-butanol ratio of 1:1 and initial pH 7.0. The molecular mass of the purified laccase secreted by analyzed strain T. versicolor was found for ≈ 66 kDa. The results showed that TPP is an efficient method for the fractionation and purification of laccase obtained from liquid cultures of T. versicolor and it allows for obtaining the relatively pure enzyme without the use of time-consuming and costly chromatographic methods.

Słowa kluczowe

EN

purification; recovery of laccase; tert-butanol; three-phase partitioning; Trametes versicolor

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2018
• Tom: Vol. 20, nr 4
• Strony: 88--95
• Opis fizyczny: Bibliogr. 31 poz., rys.

Twórcy

autor

Wasak A.

• West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Immunology, Microbiology and Physiological Chemistry, Piastów Avenue 42, 70-311 Szczecin, Poland

autor

Drozd R.

• West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Immunology, Microbiology and Physiological Chemistry, Piastów Avenue 42, 70-311 Szczecin, Poland

autor

Grygorcewicz B.

• West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Immunology, Microbiology and Physiological Chemistry, Piastów Avenue 42, 70-311 Szczecin, Poland
• Pomeranian Medical University in Szczecin, Department of Microbiology, Immunology, and Laboratory Medicine, Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland

autor

Jankowiak D.

• West Pomeranian University of Technology Szczecin, Faculty of Biotechnology and Animal Husbandry, Department of Immunology, Microbiology and Physiological Chemistry, Piastów Avenue 42, 70-311 Szczecin, Poland

autor

Rakoczy R.

• West Pomeranian University of Technology Szczecin, Faculty of Chemical Technology and Engineering, Institute Intitute of Chemical Engineering and Environmental Protection Processes, Piastów Avenue 42, 71-065 Szczecin, Poland

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).