High salt-solvent systems in separation of betanin and its derivatives from red beet (Beta vulgaris L.) by high-performance countercurrent chromatography (HPCCC)

• Autorzy: Spórna-Kucab A. , Ignatova S , Garrard I. , Wybraniec S.
• Języki publikacji: EN

Abstrakty

EN

A study on a separation of betanin and its decarboxy- and dehydro-derivatives obtained from red beet roots (Beta vulgaris L.) using analytical high-performance countercurrent chromatography (HPCCC — Dynamic Extractions Ltd., UK) was performed. The HPCCC process was accomplished in the ‘tail to head’ mode with three highly polar solvent systems with high salt concentrations: 1-propanol-acetonitrile-saturated ammonium sulphate-water (v/v/v/v, 1:0.5:1.2:1); ethanol-acetonitrile-1- propanol-saturated ammonium sulphate-water (v/v/v/v/v, 0.5:0.5:0.5:1.2:1) and ethanol-1-butanol-acetonitrile-saturated ammonium sulphate-water (volume ratio), 0.5:0.5:0.5:1.2:1). HPLC analysis was performed in a conventional reversed phase mode with diode-array (DAD) detection to characterize the composition of obtained fractions. The applied solvent systems enabled the separation of the betalain pigments with high efficiency for the first time. In the mode of separation selected, the more hydrophobic compounds eluted first as expected. Moreover, for the first time, the applied HPCCC solvent systems generated a separation of 2-decarboxy-betanin from 17- and 2,17-bidecarboxy-betanin as well as from neobetanin and betanin.

Słowa kluczowe

EN

betanin; betalains; betacyanins; countercurrent chromatography; Beta vulgaris L.

• Wydawca: Foundation for Young Scientists
• Czasopismo: Challenges of Modern Technology
• Rocznik: 2012
• Tom: Vol. 3, no. 1
• Strony: 57--61
• Opis fizyczny: Bibliogr. 43 poz., rys., tab., wykr.

Twórcy

autor

Spórna-Kucab A.

• Faculty of Analytical Chemistry, Institute C-1, Department of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland

autor

Ignatova S

• Brunel Institute for Bioengineering, Brunel University, Uxbridge, West London, UK

autor

Garrard I.

• Brunel Institute for Bioengineering, Brunel University, Uxbridge, West London, UK

autor

Wybraniec S.

• Faculty of Analytical Chemistry, Institute C-1, Department of Chemical Engineering and Technology, Cracow University of Technology, ul. Warszawska 24, Cracow 31-155, Poland

Bibliografia

• [1] Prof. Jan Bagiński, PhD, DSc, Faculty of Production Engineering, Warsaw University of Technology, Poland
• [2] Prof. Jerzy Balcerzak, PhD, DSc, Faculty of Geodesy and Cartography, Warsaw University of Technology, Poland
• [3] Prof. Zbigniew Brzózka, PhD, DSc, Faculty of Chemistry, Warsaw University of Technology, Poland
• [4] Małgorzata Celuch, PhD, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [5] Prof. Krzysztof Chełmiński, PhD, DSc, Faculty of Mathematics and Information Science, Warsaw University of Technology, Poland
• [6] Prof. Tomasz Ciach, PhD, DSc, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
• [7] Joanna Cymerys, PhD, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
• [8] Prof. Lech Czarnecki, PhD, DSc, Faculty of Civil Engineering, Warsaw University of Technology, Poland
• [9] Marta Gajadhur, PhD, Faculty of Production Engineering, Warsaw University of Technology, Poland
• [10] Prof. Wiktor Gambin, PhD, DSc, Faculty of Mechatronics, Warsaw University of Technology, Poland
• [11] Marek Gliński, PhD, Faculty of Chemistry, Warsaw University of Technology, Poland
• [12] Prof. Tadeusz Hofman, PhD, DSc Faculty of Chemistry, Warsaw University of Technology, Poland
• [12] Prof. Ewa Karwowska, PhD, DSc, Faculty of Environ mental Engineering, Warsaw University of Technology, Poland
• [13] Prof. Andrzej Kołodziejczyk, PhD, DSc, Faculty of Physics, Warsaw University of Technology, Poland
• [14] Prof. Andrzej Kraśniewski, PhD, DSc, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [15] Prof. Robert Kunkel, PhD, DSc, Faculty of Architecture, Warsaw University of Technology, Poland
• [16] Prof. Małgorzata Lewandowska, PhD, DSc, Faculty of Materials Science and Engineering, Warsaw University of Technology, Poland
• [17] Prof. Wojciech Lipiński, PhD, DSc, Faculty of Mechanical Engineering, University of Minnesota, USA
• [18] Prof. Lech Łobocki, PhD, DSc, Faculty of Environmental Engineering, Warsaw University of Technology, Poland
• [19] Prof. Roman Z. Morawski, PhD, DSc, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [20] Prof. Mieczysław Muraszkiewicz, PhD, DSc, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [21] Prof. Wiesław Nagórko, PhD, DSc, Faculty of Civil and Environmental Engineering, Warsaw University of Life Sciences, Poland
• [22] Prof. Jeremi Naumczyk, PhD, DSc, Faculty of Environmental Engineering, Warsaw University of Technology, Poland
• [23] Prof. Bożenna Pasik-Duncan, PhD, DSc, Department of Mathematics, University of Kansas, USA
• [24] Prof. Albert Podgórski, PhD, DSc, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
• [25] Marek Radomski, PhD, Faculty of Production Engineering, Warsaw University of Technology, Poland
• [26] Prof. Przemysław Rokita, PhD, DSc, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [27] Lena Ruzik, PhD, Faculty of Chemistry, Warsaw University of Technology, Poland
• [28] Prof. Jacek Senkara, PhD, DSc, Faculty of Production Engineering, Warsaw University of Technology, Poland
• [29] Prof. Alicja Smoktunowicz, PhD, DSc, Faculty of Mathematics and Information Science, Warsaw University of Technology, Poland
• [30] Prof. Tomasz Sosnowski, PhD, DSc, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
• [31] Jacek Stasierski, PhD, Faculty of Environmental Engineering, Warsaw University of Technology, Poland
• [32] Jaroslaw Yarik Syzdek, PhD, Department of Mechanical Engineering, Department of Electrical Engineering, University of California Berkeley, USA
• [33] Maciej Szwast, PhD, Faculty of Chemical and Process Engineering, Warsaw University of Technology, Poland
• [34] Iwona Wróbel, PhD, Faculty of Mathematics and Information Science, Warsaw University of Techno logy, Poland
• [35] Grzegorz Wróblewski, PhD, Faculty of Production Engineering, Warsaw University of Technology, Poland
• [36] Prof. Krzysztof Zaremba, PhD, DSc, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [37] Joanna Zarzyńska, PhD, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Poland
• [38] Justyna Zdunek-Wielgołaska, PhD, Faculty of Architecture, Warsaw University of Technology, Poland
• [39] Prof. Jaroslav Zemanek, PhD, DSc, Institute of Mathematic of the Polish Academy of Science
• [40] Prof. Małgorzata Ziarno, PhD, DSc, Faculty of Food Sciences, Warsaw University of Life Sciences, Poland
• [41] Prof. Teresa Zielińska, PhD, DSc, Faculty of Power and Aeronautical Engineering, Warsaw University of Technology, Poland
• [42] Prof. Cezary Zieliński, PhD, DSc, Faculty of Electronics and Information Technologies, Warsaw University of Technology, Poland
• [43] Prof. Wojciech Żagan, PhD, DSc, Faculty of Electrical Engineering, Warsaw University of Technology, Poland