Germination and In Vitro Propagation of Gundelia tournefontii as an Important Medicinal Plant

• Autorzy: Shatnawi Mohamad , Majdalawi Majid , Shahrour Wesam , Abu-Zahra Taleb R. , Al-Tawaha Abdel Rahman

Identyfikatory

DOI

10.12912/27197050/143006

• Języki publikacji: EN

Abstrakty

EN

Medicinal plants are important sources of medical and many other pharmaceutical goods. The conventional propagation scheme is the main means of proliferation and takes a long period of time because of poor germination and also low clonal uniformity. Rapid shoot multiplication of Gundelia tournefontii was attained from meristems on Murashige and Skoog (MS) basal medium containing 6-Benzyladenine (BAP) or kinetin, with the adding of 30 g/L sucrose. The highest number of new microshoots per explant (9.2) was attained on the MS medium enhanced with BAP and 0.05 mg/L IBA. After 13 to 14 days, microhoots started rooting on the MS medium enhanced either Indole3-butyric acid (IBA), Indole-3-acetic acid (IAA), or Naphthalene acetic acid (NAA) with the addition of 30 g/L sucrose. Using 100 to 300 ppm Gibbreline GA₃ resulted in an increase in germination percentage when soaking with GA₃ for 12 hours. Gamma radiation had a significant effect on the growth of the in vitro explants. Gamma radiation had a significant effect on the germination percentage of G. tournefontii seeds. The in vitro propagation plant of G. tournefontii plants could be used for the marketable clonal propagation of this important species or for future studies.

Słowa kluczowe

EN

Gibbreline; Gundelia tournefontii L.; medicinal plant; proliferation; root formation

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2022
• Tom: Vol. 23, iss. 1
• Strony: 57--64
• Opis fizyczny: Bibliogr. 29 poz., rys., tab.

Twórcy

autor

Shatnawi Mohamad

• Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, 19117 Al-Salt, Jordan

• https://orcid.org/0000-0003-1030-9485

autor

Majdalawi Majid

• Faculty of Zarqa, Al-Balqa Applied University, Zarqa, Jordan

autor

Shahrour Wesam

• Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, 19117 Al-Salt, Jordan

autor

Abu-Zahra Taleb R.

• Department of Plant Production and Protection, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan

autor

Al-Tawaha Abdel Rahman

• Department of Biological Sciences, Al-Hussein Bin Talal University, Maan 71111, Jordan

• https://orcid.org/0000-0001-5726-4363

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