Bacillus subtilis BS-2 and peppermint oil as biocontrol agents against Botrytis cinerea

• Autorzy: Grata Katarzyna , Rombel-Bryzek Agnieszka , Ziembik Zbigniew

Identyfikatory

DOI

10.1515/eces-2019-0044

Warianty tytułu

PL

Bacillus subtilis BS-2 oraz olejek miętowy jako czynniki biokontroli wobec Botrytis cinerea

• Języki publikacji: EN

Abstrakty

EN

The purpose of this study was to assess the activity of Bacillus subtilis BS-2 and peppermint oil against Botrytis cinerea. In this study parameters such as the age and the density of the bacterial culture and the incubation temperature were taken into consideration. Furthermore, the cellulolytic activity of the bacterium was determined. The effect of peppermint oil was evaluated at a concentration range of 0.5-4.0 %. The research was conducted with a dual culture plate method. The influence of B. subtilis BS-2 and peppermint oil on the growth of B. cinerea was evaluated based on the growth rate index. It was noted that the bacterial culture occurred at an initial density of OD ₅₆₀ = 1.0, cultivated at 30 °C for 48 hours demonstrated the strongest antagonistic effect (57.07 % inhibition). Furthermore, it was observed that the highest cellulolytic activity occurred on the bacteria incubated for 48 hours at 37 °C. The effect of mint oil, at the lowest concentration of 0.5-1.0 %, was much weaker on bacterial activity (1.1-12.1 % inhibition). The highest concentration (4.0 %) of mint oil caused the maximum inhibition (31.9 %) of the mycelial growth. B. subtilis BS-2 may be environmental-friendly alternatives for protecting plants against B. cinerea.

Słowa kluczowe

EN

Bacillus subtilis BS-2; CMCase activity; peppermint oil; antifungal activity; Botrytis cinerea

PL

Bacillus subtilis BS-2; aktywność CMCase; olej miętowy; aktywność przeciwgrzybicza; Botrytis cinerea

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. S
• Rocznik: 2019
• Tom: Vol. 26, nr 3
• Strony: 597--607
• Opis fizyczny: Bibliogr. 54 poz., wykr., tab.

Twórcy

autor

Grata Katarzyna

• Institute of Biotechnology, University of Opole, ul. kard. B. Kominka 6, 45-035 Opole, Poland, phone [phone]

autor

Rombel-Bryzek Agnieszka

• Institute of Medicine, University of Opole, ul. Oleska 48, 45-052 Opole, Poland, phone [phone]

autor

Ziembik Zbigniew

• Institute of Biotechnology, University of Opole, ul. kard. B. Kominka 6, 45-035 Opole, Poland, phone [phone]

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).