Human osteoblast-like MG 63 cells in cultures on nanofibrous PLGA membranes loaded with nanodiamonds

• Autorzy: Parizek M. , Douglas T. E. L. , Kromka A. , Renzing A. , Voss E. , Brady M. A. , Warnke P. H. , Bacakova L.
• Języki publikacji: EN

Abstrakty

EN

Using an electrospinning technique, we constructed composite nanofibrous membranes containing a copolymer of L-lactide and glycolide (PLGA, ratio 85:15) and 33 wt% of nanodiamond particles. he number of initially adhering human osteoblast-like MG 63 cells on day 1 after seeding, their spreading and subsequent growth were similar on both types of membranes. However, higher cell numbers on day 3 and 7 after seeding and a larger cell spreading area were found in the cells in the control polystyrene cell culture dishes. Nevertheless, the composite PLGA-ND membranes provided relatively good support for colonization with bone-derived cells; thus this material is promising for bone tissue engineering.

Słowa kluczowe

EN

nanofibers; nanoparticles; nanotechnology; electrospinning; bone tissue engineering

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2010
• Tom: Vol. 13, no. 94
• Strony: 11--13
• Opis fizyczny: Bibliogr. 8 poz., wykr., zdj.

Twórcy

autor

Parizek M.

• Department of Growth and Differentiation of Cell Populations, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Cz-14220 Prague 4 – Krč

autor

Douglas T. E. L.

• Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Heller-Strasse 16, 24105 Kiel, Germany

autor

Kromka A.

• Institute of Physics, Academy of Sciences of the Czech Republic, Cukrovarnicka 10, Cz-16253 Prague 6, Czech Republic

autor

Renzing A.

• Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Heller-Strasse 16, 24105 Kiel, Germany

autor

Voss E.

• Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Heller-Strasse 16, 24105 Kiel, Germany

autor

Brady M. A.

• Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Heller-Strasse 16, 24105 Kiel, Germany

autor

Warnke P. H.

• Department of Oral and Maxillofacial Surgery, University of Kiel, Arnold-Heller-Strasse 16, 24105 Kiel, Germany

autor

Bacakova L.

• Department of Growth and Differentiation of Cell Populations, Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, Cz-14220 Prague4 – Krč

Bibliografia

• [1] Webster TJ, Ergun C, Doremus RH, Siegel RW, Bizios R: Specific proteins mediate enhanced osteoblast adhesion on nanophase ceramics. J Biomed Mater Res 51: 475-483, 2000.
• [2] Price RL, Waid MC, Haberstroh KM, Webster TJ: Selective bone cell adhesion on formulations containing carbon nanofibers. Biomaterials 24:1877-1887, 2003.
• [3] Pamula E, Bacakova L, Filova E, Buczynska J, Dobrzynski P, Noskova L, Grausova L: The influence of pore size on colonization of poly(L-lactide-glycolide) scaffolds with human osteoblast-like MG 63 cells in vitro. J Mater Sci Mater Med 19: 425-435, 2008.
• [4] Pamula E, Filová E, Bacáková L, Lisá V, Adamczyk D. Resorbable polymeric scaffolds for bone tissue engineering: the influence of their microstructure on the growth of human osteoblast-like MG 63 cells. J Biomed Mater Res A 89: 432-443, 2009.
• [5] Grausova L, Bacakova L, Kromka A, Potocky S, Vanecek M, Nesladek M, Lisa V: Nanodiamond as a promising material for bone tissue engineering. J Nanosci Nanotechnol 9: 3524-3534, 2009.
• [6] Kromka A, Grausova L, Bacakova L, Vacik J, Rezek B, Vanecek M, Williams OA, Haenen K. Semiconducting to metallic-like boron doping of nanocrystalline diamond films and its effect on osteoblastic cells. Diamond Relat Mater 19: 190-195, 2010.
• [7] Mitura S, Mitura K, Niedzielski P, Louda P, Danilenko V: Nanocrystalline diamond, its synthesis, properties and applications. J AMME 16: 9-16, 2006.
• [8] Bashur CA, Dahlgren LA, Goldstein AS: Effect of fiber diameter and orientation on fibroblast morphology and proliferation on electrospun poly(D,L-lactic-co-glycolic acid) meshes. Biomaterials 27: 5681-5688, 2006.