Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells

• Autorzy: Jedrzejczak-Silicka M.

Identyfikatory

DOI

10.1515/pjct-2017-0004

• Języki publikacji: EN

Abstrakty

EN

The study was aimed at investigating the effect of the Fe₃O₄  hybrid deposited on graphene oxide (GO- Fe₃O₄) on the relative viability and DNA integrity. The properties of the GO-Fe₃O₄  hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe₃O₄  nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe₃O₄  hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe₃O₄ hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.

Słowa kluczowe

EN

GO-Fe3O4 hybrid; relative viability; DNA fragmentation; micronucleus assay; genotoxicity

• Wydawca: West Pomeranian University of Technology. Publishing House
• Czasopismo: Polish Journal of Chemical Technology
• Rocznik: 2017
• Tom: Vol. 19, nr 1
• Strony: 27--33
• Opis fizyczny: Bibliogr. 20 poz., rys.

Twórcy

autor

Jedrzejczak-Silicka M.

• West Pomeranian University of Technology, Szczecin, Laboratory of Cytogenetics, Doktora Judyma 6, 71-466 Szczecin, Poland

Bibliografia

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Uwagi

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę (zadania 2017).