A simple, rapid, sensitive and eco-friendly LC-MS/MS method for simultaneous determination of free cordycepin and isocordycepin in 10 different kinds of Cordyceps

• Autorzy: Li Wenqing , Qian Zhengming , Zou Yuansheng , Tan Guoying , Li Wenjia , Lei Qinggui , Li Runfeng , Lan Dongming

Identyfikatory

DOI

10.1556/1326.2022.01094

• Języki publikacji: EN

Abstrakty

EN

A simple, rapid, sensitive and eco-friendly liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for simultaneous determination of free cordycepin (3′-deoxyadenosine) and isocordycepin (2′-deoxyadenosine) in 10 kinds of Cordyceps samples. The samples were prepared by ultrasonic extraction at 75 °C for 30 min with boiling water as the extraction solvent. The LC separation was performed on an Agilent poroshell 120 SB-Aq C18 column (3.0 × 50 mm, 2.7 μm) in isocratic mode with an eco-friendly mobile phase (2% ethanol containing 0.2% acetic acid) at a flow rate of 0.6 mL min⁻¹, and detected by MS/MS in positive mode with multiple reaction monitoring (MRM). The developed method showed good linearity (r > 0.9990), sensitivity (LODs = 0.04 pg, LOQ = 0.1 pg), precision (RSD ≤ 3.8%) and stability (RSD ≤ 3.6%). The recoveries of developed method were 94.4–109.5% (RSD ≤ 5.5%). Compared with reported methods, the current method was rapid (less than 35% analytical time), sensitive (more than 5 folds), and eco-friendly (less than 10 μL harmful organic solvent). 10 different kinds of Cordyceps samples (40 batches) were tested by the developed method. Codycepin was only found in Cordyceps millitaris and C. millitaris fruiting body, and isocordycepin was detected in Cordyceps sinensis and other 6 Cordyceps samples. The developed method would be an improved method for the quality evaluation of Cordyceps samples.

Słowa kluczowe

EN

rapid; sensitive; LC-MS/MS; cordycepin; isocordycepin; Cordyceps

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2024
• Tom: Vol. 36, no. 1
• Strony: 59--66
• Opis fizyczny: Bibliogr. 26 poz., tab., wykr.

Twórcy

autor

Li Wenqing

• School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China

autor

Qian Zhengming

• College of Medical Imaging Laboratory and Rehabilitation, Xiangnan University, Chenzhou, 423000, PR China
• Dongguan HEC Cordyceps R&D Co., LTD, Dongguan, Guangdong, 523850, PR China

• https://orcid.org/0000-0002-8467-2941

autor

Zou Yuansheng

• Dongguan HEC Cordyceps R&D Co., LTD, Dongguan, Guangdong, 523850, PR China

autor

Tan Guoying

• Dongguan HEC Cordyceps R&D Co., LTD, Dongguan, Guangdong, 523850, PR China

autor

Li Wenjia

• Dongguan HEC Cordyceps R&D Co., LTD, Dongguan, Guangdong, 523850, PR China

autor

Lei Qinggui

• Dongguan HEC Cordyceps R&D Co., LTD, Dongguan, Guangdong, 523850, PR China

autor

Li Runfeng

• State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510000, PR China

autor

Lan Dongming

• School of Food Science and Engineering, South China University of Technology, Guangzhou, 510640, PR China

• https://orcid.org/0000-0003-2988-4703

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