Determination and pharmacokinetics of calycanthine in rat plasma by UPLC-MS/MS

• Autorzy: Lu Meifei , Lu Xiaojie , Yu Zheng , Wen Congcong

Identyfikatory

DOI

10.1556/1326.2020.00772

• Języki publikacji: EN

Abstrakty

EN

Calycanthine is an important class of alkaloids extracted and isolated from the roots, leaves, flowers and fruits of Chimonanthus praecox. In this work, the UPLC-MS/MS method was used for determination of calycanthine in rat plasma, and the pharmacokinetics in rats were investigated. Midazolam was used as an internal standard (IS), and methanol precipitation method was used to pretreatment the rat plasma samples. Chromatographic separation was achieved on a UPLC BEH C18 (50 3 2.1 mm, 1.7 mm) column with the mobile phase of methanol- 0.1% formic acid aqueous solution with gradient elution. Multiple reaction monitoring (MRM) mode with positive ionization was applied for quantitative analysis, m/z 347.3 → 246.7 and 326.2 → 291.4 for calycanthine and IS, respectively. The results indicated that within the range of 1–200 ng/mL, linearity of calycanthine in rat plasma was good (r > 0.995), and the lower limit of quantification (LLOQ) was 1 ng/mL. Accuracy range was between 90.6 and 109.4%, precision (RSD) of calycanthine was less than 14%. The matrix effect was between 97.9% and 105.4%, the recovery was better than 85.6%. The developed UPLC-MS/MS method was successfully applied in the pharmacokinetics of calycanthine in rats after oral and intravenous administration. The absolute bioavailability of the calycanthine was 37.5% in rats.

Słowa kluczowe

EN

pharmacokinetics; rat; calycanthine; UPLC-MS/MS; bioavailability

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2021
• Tom: Vol. 33, no. 1
• Strony: 91--95
• Opis fizyczny: Bibliogr. 28 poz., rys., tab.

Twórcy

autor

Lu Meifei

• Department of Pharmacy, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, PR China

autor

Lu Xiaojie

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, PR China

autor

Yu Zheng

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, PR China

autor

Wen Congcong

• Laboratory Animal Centre, Wenzhou Medical University, Wenzhou, PR China

• https://orcid.org/0000-0001-8413-1697

Bibliografia

• 1. Li, S.; Zou, Z. Pestic. Biochem. Physiol. 2019, 160, 136–45.
• 2. Shu, R. G.; Wan, Y. L.; Wang, X. M. Chin. J. Nat. Med. 2019, 17, 161–86.
• 3. Lou, H. Y., Zhang, Y.; Ma, X. P.; Jiang, S.; Wang, X. P.; Yi, P.; Liang, G. Y.; Wu, H. M.; Feng, J.; Jin, F. Y.; Pan, W. D. Chin. J. Nat. Med. 2018, 16, 621–7.
• 4. Liu, G.; Fu, J. Bot. Stud. 2018, 59, 30.
• 5. Li, Z., Jiang, Y.; Liu, D.; Ma, J.; Li, J.; Li, M.; Sui, S. Int. J. Mol. Sci. 2018, 19.
• 6. Huang, W. P., Tan, T.; Li, Z. F.; OuYang, H.; Xu, X.; Zhou, B.; Feng, Y. L. J. Pharm. Biomed. Anal. 2018, 154, 236–44.
• 7. Chen, H., Xiong, L.; Wang, N.; Liu, X.; Hu, W.; Yang, Z.; Jiang, Y.; Zheng, G.; Ouyang, K.; Wang, W. Food Funct. 2018, 9, 4959–67.
• 8. Tan, T., Luo, Y.; Zhong, C. C.; Xu, X.; Feng, Y. J. Pharm. Biomed. Anal. 2017, 141, 140–8.
• 9. Sun, Q., Zhu, J.; Cao, F.; Chen, F. PLoS One 2017, 12, e0181094.
• 10. Chen, H., Ouyang, K.; Jiang, Y.; Yang, Z.; Hu, W.; Xiong, L.; Wang, N.; Liu, X.; Wang, W. Int. J. Biol. Macromol. 2017, 98, 829–36.
• 11. Zhang, J. W., Gao, J. M.; Xu, T.; Zhang, X. C.; Ma, Y. T.; Jarussophon, S.; Konishi, Y. Chem. Biodivers. 2009, 6, 838–45.
• 12. Li, Q. J., Wang, M. L.; Yang, X. S.; Ma, L.; Hao, X.J. J. Asian. Nat. Prod. Res. 2013, 15, 270–5.
• 13. Chebib, M., Duke, R. K.; Duke, C. C.; Connor, M.; Mewett, K. N.; Johnston, G. A. Toxicol. Appl. Pharmacol. 2003, 190, 58–64.
• 14. Tian, W., Cai, J.; Xu, Y.; Luo, X.; Zhang, J.; Zhang, Z.; Zhang, Q.; Wang, X.; Hu, L.; Lin, G. Int. J. Clin. Exp. Med. 2015, 8, 15164–72.
• 15. Ma, J., Wang, S.; Huang, X.; Geng, P.; Wen, C.; Zhou, Y.; Yu, L.; Wang, X. J. Pharm. Biomed. Anal. 2015, 111, 131–7.
• 16. Hu, L. M., Zhang, M. M.; Qu, M. X.; Wang, X.; Wang, C. J.; Chen, C. M.; Zhou, Y. F. Lat. Am. J. Pharm. 2015, 34, 1252–7.
• 17. Zhang, Q., Wen, C.; Xiang, Z.; Ma, J.; Wang, X. J. Pharm. Biomed. Anal. 2014, 90, 134–8.
• 18. Huang, T., Liu, Y.; Zhang, C. Eur. J. Drug. Metab. Pharmacokinet. 2019, 44, 159–68.
• 19. Zimmer, D. Bioanalysis 2014, 6, 13–9.
• 20. FDA. Guidance for Industry Analytical Procedures and Methods Validation for Drugs and Biologics, 2014. https://www.fdanews. com/ext/resources/files/02/02-19-14-Guidance.pdf.
• 21. Cai, J. Z., Ma, J. S.; Xu, K. Q.; Gao, G.; Xiang, Y. Y.; Lin, C. L. Int. J. Clin. Exp. Med. 2015, 8, 9737–43.
• 22. Lei, X. W., Zhou, G. Z.; Fan, L. Y.; Gu, K. W.; Hu, Y.; Zhang, M. L.; Yuan, Q.; Xu, H. Q.; Zhang, K. J.; Ma, J. S. Lat. Am. J. Pharm. 2016, 35, 551–7.
• 23. Tong, S. H., Guo, J. Y.; Song, H. C.; Lv, S. W.; Zhu, Y. L.; Ma, J. S.; Zheng, Y. C. Int. J. Clin. Exp. Med. 2015, 8, 9716–22.
• 24. Wang, J. J., Wang, Z. B.; Chen, C.; Wang, Z. Y.; Liu, N. H.; Ma, J. S.; Wang, X. Q.; Hu, L. F. Lat. Am. J. Pharm. 2012, 31, 240–4.
• 25. Wang, Y., Cai, J. Z.; Fang, C. B.; Zheng, H. L.; Xu, X.; Chen, D. R.; Zhu, Q.; Zhang, Y. Y.; Zhang, X.; Ma, J. S.; Zhou, H. Int. J. Clin. Exp. Med. 2016, 9, 3593–9.
• 26. Zhang, X. H., Ma, J. S.; Hu, L. F.; Zhang, Q. W.; Wang, X. Q.; Pan, J. C.; Ye, F. Q. J. Liq. Chromatogr. Relat. Technol. 2012, 35, 1627–37.
• 27. Chen, L. G., Su, K.; Jiang, Y. Y.; Chen, B. B.; Wen, C. C.; Zeng, M.; Wang, X. C. Lat. Am. J. Pharm. 2016, 35, 1450–3.
• 28. Wang, X., Zheng, M.; Liu, J.; Huang, Z.; Bai, Y.; Ren, Z.; Wang, Z.; Tian, Y.; Qiao, Z.; Liu, W.; Feng, F. J. Ethnopharmacol. 2017, 209, 175–83.

Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2021).