Gold and silver nanoparticles in Spirulina platensis biomass for medical application

• Autorzy: Kalabegishvili T. , Murusidze I. , Kirkesali E. , Rcheulishvili A. , Ginturi E. , Kuchava N. , Bagdavadze N. , Gelagutashvili E. , Frontasyeva M. V. , Zinicovscaia I. , Pavlov S. S. , Dmitriev A. Y.

Warianty tytułu

PL

Biomasa Spirulina platensis z nanocząstkami złota i srebra do zastosowań medycznych

• Języki publikacji: EN

Abstrakty

EN

The synthesis of gold and silver nanoparticles by the blue-green algae Spirulina platensis for medical purposes was studied. A complex of optical and analytical methods was used in order to characterize produced nanoparticles. It was shown that the extracellular formation of metal nanoparticles of spherical shape with sizes in the range between 8 and 40 nm (the average size of 20-30 nm) takes place. The characteristics of gold and silver nanoparticles in the Spirulina biomass were compared. The role of biosorption processes in the synthesis of nanoparticles was estimated by using equilibrium dialysis. A positive influence of sonication on the process of microbial synthesis and yield of nanoparticles were demonstrated. The neutron activation analysis and the atomic absorption spectrometry were applied for characterizing the dynamics of gold and silver nanoparticles formation in the Spirulina platensis biomass. The neutron activation analysis was used for studying the elemental content of the Spirulina platensis biomass.

PL

Zbadano syntezę nanocząstek złota i srebra przez niebieskozielone glony Spirulina platensis, które są wykorzystywane do celów medycznych. Do scharakteryzowania wytworzonych nanocząstek zastosowano szereg metod optycznych i analitycznych. Wykazano, że zachodzi tworzenie pozakomórkowej, sferycznej nanocząstki o rozmiarach w zakresie od 8 do 40 nm (średnia wielkość 20-30 nm). Porównano charakterystyki nanocząstek złota i srebra wytworzonych w biomasie Spiruliny. Do oceny roli procesów biosorpcji w syntezie nanocząstek wykorzystano dializy równowagowe. Wykazano pozytywny wpływ ultradźwięków na procesy mikrobiologiczne i na wydajność syntezy nanocząstek. Do określenia dynamiki tworzenia nanocząsteczek złota i srebra w biomasie Spirulina platensis zastosowano neutronową analizę aktywacyjną i spektrometrię absorpcji atomowej. Stężenia pierwiastków w biomasie Spirulina platensis określono za pomocą neutronowej analizy aktywacyjnej.

Słowa kluczowe

EN

gold; silver; nanoparticles; Spirulina platensis; biotechnology; optical and analytical methods; ultrasound

PL

złoto; srebro; nanocząstki; Spirulina platensis; biotechnologia; metody optyczne i analityczne; ultradźwięki

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. S
• Rocznik: 2013
• Tom: Vol. 20, nr 4
• Strony: 621--631
• Opis fizyczny: Bibliogr. 26 poz., fot., wykr., rys., il.

Twórcy

autor

Kalabegishvili T.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia
• Ilia State University, 3/5 K. Cholokashvili Ave., Tbilisi 0162, Georgia

autor

Murusidze I.

• Ilia State University, 3/5 K. Cholokashvili Ave., Tbilisi 0162, Georgia

autor

Kirkesali E.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia
• Joint Institute for Nuclear Research, 6 Joliot-Curie Str., 1419890, Dubna, Russia

autor

Rcheulishvili A.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia

autor

Ginturi E.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia

autor

Kuchava N.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia

autor

Bagdavadze N.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia

autor

Gelagutashvili E.

• E. Andronikashvili Institute of Physics, I. Javakhishvili State University, 6 Tamarashvili Str., Tbilisi 0177, Georgia

autor

Frontasyeva M. V.

• Joint Institute for Nuclear Research, 6 Joliot-Curie Str., 1419890, Dubna, Russia
• The Institute of Chemistry of the ASM, 3 Academiei Str., 2028 Chisinau, Moldova

autor

Zinicovscaia I.

• Joint Institute for Nuclear Research, 6 Joliot-Curie Str., 1419890, Dubna, Russia

autor

Pavlov S. S.

• Joint Institute for Nuclear Research, 6 Joliot-Curie Str., 1419890, Dubna, Russia

autor

Dmitriev A. Y.

• Joint Institute for Nuclear Research, 6 Joliot-Curie Str., 1419890, Dubna, Russia

Bibliografia

• [1] Salata OV. J Nanobiotechnol. 2004;2:1-6. DOI:10.1186/1477-3155-2-12.
• [2] Li X, Xu H, Chen ZS, Chen G. J Nanomat.2011;1-16. DOI:10.1155/2011/270974.
• [3] Beverige TJ. Int Rev Cytol. 1981;72:229-317.
• [4] Hirsch LR, Stafford RJ, Bankson JA, Sershen SR, Rivera B, Price RE, et al. Proc Natl Acad Sci USA. 2003;100:13549-135554. DOI:10.1073/pnas.2232479100.
• [5] Dykman LA, Khlebtsov NG. Acta Naturae. 2011:3:34-55. PMID:22649683.
• [6] Singh M, Singh S, Prasad S, Gambhir IS. Dig J Nanomater Bios. 2008;3:115-122.
• [7] Ahamed M, Alsalhi MS, Siddiqui MK. Clin Chim Acta. 2010;411:1841-1848. PubMed: 20719239.
• [8] Kim CJ, Jung YH, Oh HM. J Microbiol. 2007;45:122-127.
• [9] Belay A, Ota Y, Miyakawa K, Shimamatsu H. J Appl Phycol. 1993;5:235-241.
• [10] Doshi H, Ray A, Kothari IL. Biotechnol Bioeng. 2007;96;1051-1063. DOI 10.1002/bit.21190.
• [11] Sadowski Z. Biosynthesis and Application of Silver and Gold Nanoparticles. In: Pozo Perez D, editor. Silver nanoparticles. InTech; 2010. DOI: 10.5772/8508.
• [12] Govindaraju K, Basha SK, Kumar VG, Singaravelu G, J Mater Sci. 2008;43:5115-5122.
• [13] Mosulishvili LM, Belokobylsky AI, Kirkesali EI, Frontasyeva MV, Pavlov SS, Aksenova NG. J Neutron Res. 2007;15: 49-54. DOI: 10.1080/10238160601025138.
• [14] Mosulishvili LM, Frontasyeva MV, Pavlov SS, Belokobylsky AI, Kirkesali EI, Khizanishvili AI, et al.J Pharm Biomed Anal. 2002;30:87-97. PubMed PMID: 12151068.
• [15] Mosulishvili LM, Belokobilsky AI, Kirkesali EI, Frontasyeva MV, Pavlov SS. Method of production of chromium-containing pharmaceuticals based on blue-green algae Spirulina platensis. R. F. Patent No 2230560, June 11; 2002.
• [16] Mosulishvili LM, Belokobylsky AI, Khizanishvili AI, Kirkesali EI, Frontasyeva MV, Pavlov SS. Method of production of selenium-containing pharmaceuticals based on blue-green algae Spirulina platensis. R. F.Patent No 2209077, March 15; 2001.
• [17] Tsibakhashvili NY, Kirkesali EI, Pataraya DT, Gurielidze MA, Kalabegishvili TL, Gvarjaladze DN, et al.Adv Sci Let. 2011;4:3408-3417.
• [18] Kalabegishvili T, Kirkesali E, Rcheulishvili A, Ginturi E, Murusidze I, Kuchava N, et al. Adv Sci Eng Med. 2013;5:30-36.
• [19] Mosulishvili LM, Nadareishvili VS, Kharabadze NE, Belokobylsky AI. Facility for Lyophilization of Biological Preparation. URSS Patent No 779765, Bul. 42; 1980.
• [20] Dmitriev AYu, Pavlov SS. Particles and Nuclei, Letters. 2013;10:58-64.
• [21] Cullity BD, Stock SR. Elements of X-Ray Diffraction. 3rd Ed. Prentice-Hall Inc.; 2001;167-171.
• [22] Jenkins R, Snyder RL. Introduction to X-ray Powder Diffractometry. John Wiley & Sons Inc.; 1996;89-91.
• [23] van Hullebusch ED, Zandvoort MH, Lens PNL. Rev Environ Sci Biotechnol. 2003;2:9-33.
• [24] Sosa IO, Noguez C, Barrera PG. J Phys Chem B. 2003;107:6269-6275.
• [25] Pimprikar S, Joshi SS, Kumar ARR, Zinjarde SS. Kulkarni SK. Colloid Surface B. 2009;74:309-316.
• [26] W.H.O. Elements in human nutrition and health, Geneva: WHO; 1996.