Assessing the efficacies of phenolic compounds in pomegranate juice using thin-layer chromatography

• Autorzy: Fakhri E. , Petróczi A. , Naughton D. P.
• Języki publikacji: EN

Abstrakty

EN

Pomegranates (Punica granatum) have been known for centuries for their healing properties. The phenolic components of pomegranate are believed to be responsible for their antioxidant activities, hence playing a major role in reducing oxidative stress-related disease, such as cardiovascular diseases, cancer, and neurodegenerative diseases. In this study, thin-layer chromatography (TLC) was used to, first, separate and identify the phenolic constituents of pomegranate juice and, second, to assess the antioxidant efficacies of the identified compounds. Different oxidant and hydroxyl radical generating systems (Fe3+, Cu2+, H2O2, Fe2+-H2O2, and Cu2+-H2O2) were used in assessing the efficacies of phenolic compounds found in pomegranate juice. A 10 × 10 cm and 20 × 20 cm sized silica gel 60 F254 TLC plates with toluene-ethyl acetate-formic acid (60:40:10 v/v/v) as a mobile phase were used for the chromatographic separation. Two compounds, ellagic acid and gallic acid, were separated and identified. When pomegranate juice was challenged with the oxidant systems, it was observed that the phenolic compounds slowly disappeared in a concentration- and time-dependent manner. From the results, it was concluded that gallic acid had a higher antioxidant efficacy than ellagic acid. TLC has been applied for the first time to outline the antioxidant profile of pomegranate juice and assess the efficacies of phenolics using different oxidant systems, including redox-active metals and H2O2.

Słowa kluczowe

EN

pomegranate juice; thin-layer chromatography; hydrogen peroxide; metal ions; antioxidants

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 3
• Strony: 563--573
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Fakhri E.

• Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK

autor

Petróczi A.

• Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK

autor

Naughton D. P.

• Kingston University School of Life Sciences Kingston upon Thames London KT1 2EE UK

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