Nowoczesne metody badania bioróżnorodności biocenoz bakteryjnych w środowisku

• Autorzy: Karło A. , Ziembińska A.

Warianty tytułu

EN

Modern techniques used for biodiversity analysis in bacterial environmental communities

• Konferencja: XIX Konferencja Przemysł Chemiczny : wyzwania i bariery z cyklu restrukturyzacja
• Języki publikacji: PL EN

Abstrakty

PL

W analizach biocenoz bakteryjnych coraz powszechniej wykorzystywane są techniki biologii molekularnej. Wśród nich można wyróżnić metodę FISH – fluorescencyjnej hybrydyzacji in situ oraz jej modyfikacje (RING-FISH, Clone-FISH, CARDFISH i MAR-FISH). W artykule opisano też technikę elektroforezy w gradiencie denaturacji (DGGE), bazującą na bakteryjnym markerze molekularnym 16S rRNA oraz cystometrię przepływową. Ze względu na dużą przewagę tych metod nad klasycznymi metodami mikrobiologicznymi, wynikającą między innymi z możliwości analizowania próbek pobieranych bezpośrednio ze środowiska, są one stosowane w biotechnologii środowiskowej, np. w badaniach bakteryjnej biocenozy osadu czynnego, biorącej udział w biologicznym oczyszczaniu ścieków. Możliwe jest również użycie kilku metod, których rezultaty są komplementarne i pozwalają na stworzenie całościowego obrazu badanej biocenozy.

EN

Molecular techniques are very popular in microbial laboratories. Among them FISH- fluorescent in situ hybridization (with modifications like: CARD-FISH, MAR-FISH, RING-FISH, Clone- FISH), DGGE – denaturing gradient gel electrophoresis based on 16S rRNA molecular marker and flow cytometry, are the most popular. These analytical methods are commonly use in bacterial biodiversity research. The analysis can be performed directly on the environmental sample, so these procedures are simpler and faster that traditional ones. Therefore, molecular techniques can be used in aqueous bacterial biocenosis research, such as activated sludge, which takes part in biological wastewater treatment. It is also possible to use a set of molecular methods in order to obtain complimentary results to present total bacterial biocenosis picture.

Słowa kluczowe

PL

osad czynny; FISH; DGGE; cytometria przepływowa; bioróżnorodność bakterii

EN

activated sludge; FISH; DGGE; flow cytometry; bacterial biodiversity

• Wydawca: Stowarzyszenie Inżynierów i Techników Przemysłu Chemicznego, ZW CHEMPRESS-SITPChem
• Czasopismo: Chemik
• Rocznik: 2013
• Tom: Vol. 67, nr 11
• Strony: 1105--1114
• Opis fizyczny: Bibliogr. 44 poz., tab., rys.

Twórcy

autor

Karło A.

• Katedra Biotechnologii Środowiskowej, Politechnika Śląska, Gliwice

autor

Ziembińska A.

• Katedra Biotechnologii Środowiskowej, Politechnika Śląska, Gliwice

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