Verification of biological properties of poly-ε-caprolacton (PCL) as the material dedicated to medicine and biotechnology

• Autorzy: Wieczorek J. , Grad I. , Stodolak-Zych E. , Kosenyuk Y.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was evaluation of possibility of poly-ε-caprolacton (PCL) application as potential material for production of medical devices, as catheters for obtaining and transporting of embryos as well as dishes for embryos culture in vitro and covers for cryoconservation. The possible application of this biomaterial needs verification of its biological properties on embryos culture. The foil discs made of policaprolacton, thickness 0.5 mm, diameter 3.5 mm, were prepared in two forms: the baseline one (nPCL) and thermally modified by freezing with liquid nitrogen (mPCL). The verification of PCL bioconcordance was performed by evaluation of 102 pig embryos. To evaluate poly-ε-caprolacton bioconcordance we performed 5-day long culture of embryos on the evaluated material, not frozen (nPCL) and frozen in liquid nitrogen (mPCL) and additionally culture after short contact with poly-ε-caprolacton, lasting 15 minutes (nPCL-15). In all evaluated study groups the development of embryos was suppressed shortly after transfer to the culture with PCL. In the control group. 74%-78% of embryos reached blastocyst stage. Polycaprolacton cannot be used as the material for catheter production used in biotechnology of animal reproduction and other materials used for in vitro culture and cryoconservation.

Słowa kluczowe

EN

embryos; poly(ε-caprolactone) (PCL); culture; catheters; pigs

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2013
• Tom: Vol. 16, no. 122-123 spec. iss.
• Strony: 66--68
• Opis fizyczny: Bibliogr. 13 poz., tab.

Twórcy

autor

Wieczorek J.

• National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 1 Krakowska str., Balice, Poland

autor

Grad I.

• National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 1 Krakowska str., Balice, Poland

autor

Stodolak-Zych E.

• AGH University of Science and Technology, Faculty of Materials Science and Ceramics, Department of Biomaterials, 30 Mickiewicz ave, 30-059 Krakow, Poland

autor

Kosenyuk Y.

• National Research Institute of Animal Production, Department of Biotechnology of Animal Reproduction, 1 Krakowska str., Balice, Poland

Bibliografia

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Uwagi

EN

This work was financially supported by the Polish Ministry of Science and Higher Education project number: N N302 632140