A simple TLC-MALDI method to monitor oxidation products of phosphatidylcholines and -ethanolamines

• Autorzy: Bischoff A , Eibisch M , Fuchs B , Süss R , Schürenberg M , Suckau D , Schiller J.
• Języki publikacji: EN

Abstrakty

EN

The analysis of lipid-phospholipid oxidation products is of primary importance. Although there are established HPLC and LC-MS techniques, it is shown here for the first time that the combination of matrix-assisted laser desorption and ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) and TLC represents a sensitive, fast, and convenient alternative. A mixture of 1-palmitoyl-2-linoleoyl-sn-phosphatidylcholine (PLPC) and -ethanolamine (PLPE) was oxidized under the influence of atmospheric oxygen and characterized by direct positive ion MALDI-TOF MS as well as combined TLC-MALDI. It is shown that much more detailed information — particularly related to the oxidation products of PLPE that have so far been scarcely investigated — can be obtained by TLC-MALDI. However, it is also shown that further methodological improvements are necessary to make this method generally applicable to complex lipid mixtures.

Słowa kluczowe

EN

phosphatidylethanolamine; phosphatidylcholine; liver; air oxidation; MALDI-TOF MS; thin-layer chromatography; TLC-MALDI

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2011
• Tom: Vol. 23, no. 2
• Strony: 365--375
• Opis fizyczny: Bibliogr. 24 poz., rys., tab.

Twórcy

autor

Bischoff A

• Faculty of Medicine Institute of Medical Physics and Biophysics D-04107 Leipzig Germany

autor

Eibisch M

• Faculty of Medicine Institute of Medical Physics and Biophysics D-04107 Leipzig Germany

autor

Fuchs B

• Faculty of Medicine Institute of Medical Physics and Biophysics D-04107 Leipzig Germany

autor

Süss R

• Faculty of Medicine Institute of Medical Physics and Biophysics D-04107 Leipzig Germany

autor

Schürenberg M

• Bruker Daltonik GmbH D-28359 Bremen Germany

autor

Suckau D

• Bruker Daltonik GmbH D-28359 Bremen Germany

autor

Schiller J.

• Faculty of Medicine Institute of Medical Physics and Biophysics D-04107 Leipzig Germany

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