The chemical digestion of Ti6Al7Nb scaffolds produced by Selective Laser Melting reduces significantly ability of Pseudomonas aeruginosa to form biofilm

• Autorzy: Junka A. F. , Szymczyk P. , Secewicz A. , Pawlak A. , Smutnicka D. , Ziółkowski G. , Bartoszewicz M. , Chlebus E.

Identyfikatory

DOI

10.5277/ABB-00333-2015-01

• Języki publikacji: EN

Abstrakty

EN

In our previous work we reported the impact of hydrofluoric and nitric acid used for chemical polishing of Ti-6Al-7Nb scaffolds on decrease of the number of Staphylococcus aureus biofilm forming cells. Herein, we tested impact of the aforementioned substances on biofilm of Gram-negative microorganism, Pseudomonas aeruginosa, dangerous pathogen responsible for plethora of implant-related infections. The Ti-6Al-7Nb scaffolds were manufactured using Selective Laser Melting method. Scaffolds were subjected to chemical polishing using a mixture of nitric acid and fluoride or left intact (control group). Pseudomonal biofilm was allowed to form on scaffolds for 24 hours and was removed by mechanical vortex shaking. The number of pseudomonal cells was estimated by means of quantitative culture and Scanning Electron Microscopy. The presence of nitric acid and fluoride on scaffold surfaces was assessed by means of IR and rentgen spetorscopy. Quantitative data were analysed using the Mann–Whitney test (P ≤ 0.05). Our results indicate that application of chemical polishing correlates with significant drop of biofilm-forming pseudomonal cells on the manufactured Ti-6Al-7Nb scaffolds ( p = 0.0133, Mann–Whitney test) compared to the number of biofilm-forming cells on non-polished scaffolds. As X-ray photoelectron spectroscopy revealed the presence of fluoride and nitrogen on the surface of scaffold, we speculate that drop of biofilm forming cells may be caused by biofilm-supressing activity of these two elements.

Słowa kluczowe

EN

SLM technology; Pseudomonas aeruginosa; scaffolds; surface modification; Ti-6Al-7Nb

PL

technologia SLM; Pseudomonas aeruginosa; skafoldy; modyfikacja powierzchni; Ti-6Al-7Nb

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Acta of Bioengineering and Biomechanics
• Rocznik: 2016
• Tom: Vol. 18, no. 1
• Strony: 115--120
• Opis fizyczny: Bibliogr. 19 poz., rys., tab., wykr.

Twórcy

autor

Junka A. F.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wrocław, Poland

autor

Szymczyk P.

• Centre for Advanced Manufacturing Technologies, Wrocław University of Science and Technology, Wrocław, Poland

autor

Secewicz A.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wrocław, Poland

autor

Pawlak A.

• Centre for Advanced Manufacturing Technologies, Wrocław University of Science and Technology, Wrocław, Poland

autor

Smutnicka D.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wrocław, Poland

autor

Ziółkowski G.

• Centre for Advanced Manufacturing Technologies, Wrocław University of Science and Technology, Wrocław, Poland

autor

Bartoszewicz M.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wrocław, Poland

autor

Chlebus E.

• Department of Pharmaceutical Microbiology and Parasitology, Medical University of Wrocław, Poland

Bibliografia

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Uwagi

PL

Opracowanie ze środków MNiSW w ramach umowy 812/P-DUN/2016 na działalność upowszechniającą naukę.