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Development and validation of HPTLC method for estimation of gymnemic acid in microencapsulated antidiabetic polyherbal formulations

Identyfikatory
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Gymnemic acid (GA) is one of the phytoconstituents present in Gymnema sylvestre. Estimation of GA was carried out first time from microencapsulated polyherbal formulation. Microencapsulated polyherbal formulations (F1 and F2) contain various plant extracts; hence, proper resolution of GA peak in high-performance thin-layer liquid chromatography (HPTLC) analysis of F1 and F2 is the problem. Hence, HPTLC analysis method for F1 and F2 is developed and validated for quantitative determination of GA. HPTLC analysis of F1 and F2 was carried out using TLC aluminum plates precoated with silica gel 60F254 eluted with chloroform-methanol-water (6.5 mL + 4.5 mL + 1.0 mL), and densitometric analysis was carried out at 580 nm. Complete validation was performed using standard methods. This HPTLC method was found to be reproducible, accurate, and can detect GA at microgram level. The new optimized mobile phase gave good resolution of GA peak for its proper quantification in microencapsulated polyherbal formulation.
Rocznik
Strony
601--612
Opis fizyczny
Bibliogr. 21 poz., rys., tab.
Twórcy
autor
  • Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India
autor
  • Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India
autor
  • Pravara Rural College of Pharmacy Department of Pharmacognosy Pravaranagar, Loni 413736 Maharashtra India
Bibliografia
  • [1] P.K. Mukherjee, Clin. Res. Reg. Affairs, 20, 249–264 (2003)
  • [2] Y. Jianga, B. Tu, P. David, and Y. Barbin, Anal. Chim. Acta, 657, 9–18 (2010)
  • [3] X. Anandjiwala, J. Kalola, and M. Rajani, J. AOAC Int., 89, 1467–1474 (2006)
  • [4] A.K. Nadkarni, Indian Materia Media, 3rd edn., Bombay Popular Prakashan, Bombay, 1982, pp. 116
  • [5] K.R. Kirtikar and B.D. Basu, Indian Medicinal Plants, 2nd edn., International Book Distributors, Deharadun, 1987, pp. 66–69, 536–541, 677, 2423, 2220
  • [6] E. Reich and A. Schibli, High performance thin layer chromatography for the analysis of medicinal plants, Thieme Publishers, New York, 2007, pp. 130–162
  • [7] M. Chaturvedi, M. Kumar, A. Sinhal, and A. Saifi, Int. J. Green Pharm., 5, 87–94 (2011)
  • [8] D.M. Brahmankar and S.B. Jaiswal, Biopharmaceutics and Pharmacokinetics: a Treatise, Vallabh Prakashan Pitampura, Delhi, 1st edition, 2001, pp. 337–341
  • [9] H. Patel, R. Patel, and G. Patel, Ionotropic gelation technique for microencapsulation of antihypertensive drug, http://www.webmedcentral.com/article_view/922 (2010)
  • [10] R.B. Laware, S.A. Nirmal, S.V. Joshi, S.T. Gore, B.S. Patel, A.K. Bhagat, and B.S. Kuchekar, Indian Drugs, 45, 279–287 (2008)
  • [11] ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1), Geneva, 2005, pp. 27
  • [12] R.S. Kujur, V. Singh, M. Ram, H.N. Yadava, K.K. Singh, S. Kumar, and B.K. Roy, Pharmacog. Res., 2, 258–263 (2010)
  • [13] H.A. Lawal, M.K. Atiku, D.G. Khelpai, and N.N. Wannang, Niger. J. Physiol. Sci., 23, 37–40 (2008)
  • [14] F. Martinello, S.M. Soares, J.J. Franco, A.C. Santos, A. Sugohara, S.B. Garcia, C. Curti, and S.A. Uyemura, Food Chem. Toxicol., 44, 810–818 (2006)
  • [15] B.D. Kumar, A. Mitra, and M. Manjunatha, J. Herb. Med. Toxicol., 3, 9–14 (2009)
  • [16] G.K. Mall, P.K. Mishra, and P. Veeru, Global J. Biotechnol. Biochem., 4, 37–42 (2009)
  • [17] M. Bokaeian, A. Nakhaee, B. Moodi, A. Farhangi, and A. Akbarzadeh, Indian J. Clin. Biochem., 25, 182–187 (2010)
  • [18] M. Gunjan, A.K. Jha, G.K. Jana, U. Ishra, and U. Verma, J. Pharm. Res., 3, 1223–1224 (2010)
  • [19] P.D. Trivedi and K.A. Pundarikakshudu, Int. J. Appl. Sci. Eng., 6(1), 19–28 (2008)
  • [20] U. Kumar, I. Singh, Priyanka, and Y. Vimala, Afr. J. Biotechnol., 9, 4904–4909 (2010)
  • [21] P.V. Kanetkar, R.S. Singhal, K.S. Laddha, and M.Y. Kamat, Phytochem. Anal, 17, 409–413 (2006)
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-df46ddce-f420-4165-91c1-01cb5bdc2f52
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