Comparative study of phenol degradation with a wild-type and genetically modified P. vesicularis (pBR322). plasmid stability and fame profiling

• Autorzy: Mrozik A. , Swędzioł Ż. , Miga S.

Identyfikatory

DOI

10.5277/epe150111

• Języki publikacji: EN

Abstrakty

EN

It was evidenced that P. vesicularis is an efficient degrader of phenol but does not have a reporter system for monitoring bacterial survival in the environment. Therefore, P. vesicularis (pBR322) has been constructed. In this study we experimentally confirmed that introduction of plasmid pBR322 into P. vesicularis did not change its ability to degrade phenol in liquid media and after its inoculation into sterile soil. Moreover, it has been shown that plasmid pBR322 was stable in P. vesicularis during all experiments. Additionally, the pattern of fatty acid methyl esters for P. vesicularis (pBR322) looked similar to that of P. vesicularis under phenol exposure. Some fatty acids, especially branched and cyclopropane ones were sensitive markers of phenol utilization. These findings indicate that P. vesicularis (pBR322) due to the presence of plasmid could be used instead of P. vesicularis in bioaugmentation of phenol-contaminated areas.

Słowa kluczowe

EN

biodegradation; phenols; comparative studies; fatty acid methyl ester; phenol degradation; plasmid stability

PL

biodegradacja; kwasy tłuszczowe; fenole; badania porównawcze; ester metylowy kwasu tłuszczowego; degradacja fenolu; stabilność plazmidu

• Wydawca: Oficyna Wydawnicza Politechniki Wrocławskiej
• Czasopismo: Environment Protection Engineering
• Rocznik: 2015
• Tom: Vol. 41, nr 1
• Strony: 137--155
• Opis fizyczny: Bibliogr. 25 poz., tab., rys.

Twórcy

autor

Mrozik A.

• Department of Biochemistry, University of Silesia, ul. Jagiellońska 28, 40-032 Katowice, Poland

autor

Swędzioł Ż.

• Department of Biochemistry, University of Silesia, ul. Jagiellońska 28, 40-032 Katowice, Poland

autor

Miga S.

• Institute of Materials Science, University of Silesia, ul. 75 Pułku Piechoty 1A, 41-500 Chorzów, Poland

Bibliografia

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