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We discuss the basic parameters of the holographic tweezers equipped with a diode laser and cheap video camera. We compare these parameters with the system using a fast camera and high power Nd:YAG laser. The measured parameters are: the power spectra density calculated from tracing the position of the micron polystyrene beads and the trap stiffness. We show that this cheap optical tweezers system is sufficient for experiments in microbiology.
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Tom
Strony
739--746
Opis fizyczny
Bibliogr. 11 poz., rys., wykr.
Twórcy
autor
- Institute of Physics, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland
autor
- Department of Pathology, Faculty of Medicine, Wrocław Medical University, Marcinkowskiego 1, 50-368 Wrocław, Poland
autor
- Department of Pathology, Faculty of Medicine, Wrocław Medical University, Marcinkowskiego 1, 50-368 Wrocław, Poland
autor
- Laboratory for Biophysics of Lipid Aggregates, Institute of Biomedical Engineering and Instrumentation, Wrocław University of Technology, pl. Grunwaldzki 13, 50-377 Wrocław, Poland
Bibliografia
- [1] STEVENSON D., GUNN-MOORE F., DHOLAKIA K., Light forces the pace: optical manipulation for biophotonics, Journal of Biomedical Optics 15(4), 2010, article 041503.
- [2] FARRÉ A., VAN DER HORST A., BLAB G.A., DOWNING B.P.B., FORDE N.R., Stretching single DNA molecules to demonstrate high-force capabilities of holographic optical tweezers, Journal of Biophotonics 3(4), 2010, pp. 224–233.
- [3] SINGER W., BERNET S., HECKER N., RITSCH-MARTE M., Three-dimensional force calibration of optical tweezers, Journal of Modern Optics 47(14/15), 2000, pp. 2921–2931.
- [4] BERG-SØRENSEN K., FLYVBJERG H., Power spectrum analysis for optical tweezers, Review of Scientific Instruments 75(3), 2004, pp. 594–612.
- [5] SIMMONS R.M., FINER J.T., CHU S., SPUDICH J.A., Quantitative measurements of force and displacement using an optical trap, Biophysical Journal 70(4), 1996, pp. 1813–1822.
- [6] GRIER D.G., ROICHMAN Y., Holographic optical trapping, Applied Optics 45(5), 2006, pp. 880–887.
- [7] COJOC D., GARBIN V., FERRARI E., BUSINARO L., ROMANATO F., DI FABRIZIO E., Laser trapping and micro-manipulation using optical vortices, Microelectronic Engineering 78–79, 2005, pp. 125–131.
- [8] MCGLOIN D., DHOLAKIA K., Bessel beams: diffraction in a new light, Contemporary Physics 46(1), 2005, pp. 15–28.
- [9] VAN DER HORST A., FORDE N.R., Power spectral analysis for optical trap stiffness calibration from high-speed camera position detection with limited bandwidth, Optics Express 18(8), 2010, pp. 7670–7677.
- [10] CHERNOV N., Fitting circles to scattered data: parameter estimates have no moments, Metrika 73(3), 2011, pp. 373–384.
- [11] GIBSON G.M., LEACH J., KEEN S., WRIGHT A.J., PADGETT M.J., Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy, Optics Express 16(19), 2008, pp. 14561–14570.
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Bibliografia
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