Application of ultrasound-assisted liquid-solid extraction for the isolation of PAHs from organic-rich soil samples

• Autorzy: Sołtys Agnieszka , Wideł Dariusz , Dołęgowska Sabina

Identyfikatory

DOI

10.24425/aep.2025.154756

Warianty tytułu

PL

Zastosowanie ekstrakcji ciecz-ciało stałe wspomaganej ultradźwiękami do izolacji WWA z próbek gleb bogatych w materię organiczną

• Języki publikacji: EN

Abstrakty

EN

Polycyclic aromatic hydrocarbons (PAHs) occur in soils at concentrations of ng·g^-1 or less, and their levels are influenced by a number of factors, including the content of organic matter. Extraction of PAHs from soils, enriched in organic fraction, can be problematic, time-consuming and cost-intensive. The aim of this study was to modify an ultrasound-assisted solid-liquid extraction method, used for the isolation of 16 priority PAHs from forest soils collected from soil (sub)horizons with different organic matter content. The following parameters were considered: (i) the type and volume of solvent, (ii) the time of extraction and (iii) the purification of extracts by the SPE method. The final qualitative and quantitative determination of 16 PAHs was performed by the GC-MS method. The following results were obtained: recovery 71-107%, R² = 0.993-0.999, LOD = 0.008-0.026 µg·ml^-1 and LOQ = 0.024-0.078 µg·ml^-1. The above method was successfully applied for the extraction of selected PAHs from organic soil samples collected from forest complexes located in south-central Poland.

PL

Wielopierścieniowe węglowodory aromatyczne (WWA) występują w glebach w stężeniach ng·g^-1 lub niższych, a na ich poziom wpływa szereg czynników, w tym zawartość materii organicznej. Ekstrakcja WWA z gleb wzbogaconych w materię organiczną może być problematyczna, czasochłonna i kosztowna. Celem tej pracy była modyfikacja metody ekstrakcji ciało stałe - ciecz wspomaganej ultradźwiękami, stosowanej do izolacji 16 priorytetowych WWA z gleb leśnych zebranych z (pod)poziomów glebowych o różnej zawartości materii organicznej. Podczas ustalania metodyki ekstrakcji wspomaganej ultradźwiękami z gleb leśnych bogatych w materię organiczną wzięto pod uwagę następujące parametry: (i) rodzaj i objętość rozpuszczalnika, (ii) czas ekstrakcji oraz (iii) oczyszczanie ekstraktów metodą SPE. Jakościowe i ilościowe oznaczenie 16 WWA wykonano metodą chromatografii gazowej sprzężoną ze spektrometrią mas (GC-MS). Uzyskano następujące wyniki: odzysk 71-107%, R² = 0,993-0,999, LOD = 0,008-0,026 µg·ml^-1 i LOQ = 0,024-0,078 µg·ml^-1. Powyższą metodę z powodzeniem zastosowano do ekstrakcji wybranych WWA z próbek gleb organicznych pobranych z kompleksów leśnych zlokalizowanych w środkowo-południowej Polsce.

Słowa kluczowe

EN

polycyclic aromatic hydrocarbons; gas chromatography-mass spectrometry; forest soil samples; liquid-solid extraction; ultrasound assisted

PL

wielopierścieniowe węglowodory aromatyczne; gleba leśna; ekstrakcja wspomagana ultradźwiękami; ekstrakcja ciało stałe - ciecz

• Wydawca: Institute of Environmental Engineering, Polish Academy of Sciences
• Czasopismo: Archives of Environmental Protection
• Rocznik: 2025
• Tom: Vol. 51, no. 2
• Strony: 53--61
• Opis fizyczny: Bibliogr. 26 poz., rys., tab., wykr.

Twórcy

autor

Sołtys Agnieszka

• Institute of Chemistry, Jan Kochanowski University, Kielce, Poland

autor

Wideł Dariusz

• Institute of Chemistry, Jan Kochanowski University, Kielce, Poland

autor

Dołęgowska Sabina

• Institute of Chemistry, Jan Kochanowski University, Kielce, Poland

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa nr POPUL/SP/0154/2024/02 w ramach programu "Społeczna odpowiedzialność nauki II" - moduł: Popularyzacja nauki (2025).