Targeted Cellular Tracking of Pancreatic Cancer Cells via Magnetic Particle Spectroscopy (MPS)

• Autorzy: Dinari Ali , Ahmad Hafiz Ashfaq , Bùi Minh Phú , Oh Seungjun , Kim Yun-Hee , Kim Dae-Hong , Yoon Jungwon

Identyfikatory

DOI

10.5604/01.3001.0054.9363

• Języki publikacji: EN

Abstrakty

EN

Objective: Pancreatic cancer is an asymptomatic disease and, based on statistical studies, it is the fourth leading cause of cancer-related death. Pancreatic ductal adenocarcinoma (PDAC), which accounts for over 95% of pancreatic cancers, is typically detectable at advanced stages. Standard diagnostic methods include bloodbased tests and imaging. Standard diagnostic methods include blood-based tests and imaging. Biomarkers play a key role as indicators in blood tests, offering valuable insights into disease detection and monitoring. Mesothelin, a cell-surface glycoprotein, and vimentin, an intermediate filament protein, are promising biomarker candidates. Methods: In this study, these biomarkers were conjugated with magnetic nanoparticles (MNPs) and utilized for cellular tracking through magnetic particle spectroscopy (MPS). Capan-1 (a pancreatic cancer cell line) and bone marrow stem cells (BMSC) were treated with the targeted MNPs. Subsequently, MNP-labelled cells were evaluated with imaging modalities such as MPS and confocal microscopy. Results: In the case of the MPS modality, a home-made MPS device with a detection limit of 1 ng of MNPs was used. The results showed that MPS can quantitatively trace MNPs signals and differentiate between various treatments. Conclusions: Detection of labelled cells via MPS is a novel method with features such as sensitivity, non-invasiveness, and no background noise. This new technology can pave the way for imaging quantification of pancreatic cancer in its primary stages and for tracking cancer cell populations. populations.

Słowa kluczowe

EN

cellular tracking; pancreatic cancer; mesothelin; vimentin; magnetic particle spectroscopy

• Wydawca: Uniwersytet Jagielloński - Collegium Medicum ; Index Copernicus Sp. z o.o.
• Czasopismo: Bio-Algorithms and Med-Systems
• Rocznik: 2024
• Tom: Vol. 20, Special issue
• Strony: 63--70
• Opis fizyczny: Bibliogr. 43 poz., rys., tab.

Twórcy

autor

Dinari Ali

• School of Integrated Technology, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea

autor

Ahmad Hafiz Ashfaq

• School of Integrated Technology, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea

autor

Bùi Minh Phú

• School of Integrated Technology, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea

autor

Oh Seungjun

• School of Integrated Technology, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea

autor

Kim Yun-Hee

• National Cancer Center, Goyang-si, Gyeonggi-do, Republic of Korea

autor

Kim Dae-Hong

• National Cancer Center, Goyang-si, Gyeonggi-do, Republic of Korea

autor

Yoon Jungwon

• School of Integrated Technology, Gwangju Institute of Science and Technology, Gwangju 61005, Republic of Korea

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