Preparative separation of two alkaloids from devil pepper radix (Rauvolfia verticillata [Lour.] Baill.) by ph-zone-refining counter-current chromatography

• Autorzy: Duan W. J. , Liu Q. , Zhao R. X. , Mu Y. , Guo L. P. , Li D. P. , Wang X.

Identyfikatory

DOI

10.1556/1326.2017.00269

• Języki publikacji: EN

Abstrakty

EN

A method was developed for the preparative separation of two alkaloids from the crude extract of the radix of Rauvolfia verticillata (Lour.) Baill. in a single run. The two-phase solvent system composed of petroleum ether–ethyl acetate–methanol–water (5:5:2:8, v/v), where triethylamine (40 mmol/L) was added to the upper organic phase as the stationary phase and hydrochloric acid (10 mmol/L) was added to the lower aqueous phase as the mobile phase, was selected for this separation by pH-zone-refining counter-current chromatography (PZRCCC). For the preparative separation, the apparatus was rotated at a speed 850 rpm, while the mobile phase was pumped into the column at 2 mL/min. As a result, 112 mg of reserpine and 21 mg of yohimbine were obtained from 3 g of crude extract in a single run. The analysis of the isolated compounds was determined by high-performance liquid chromatography (HPLC) at 230 nm with purities of over 91.0%, and the chemical identification was carried out by the data of electrospray ionization–mass spectrometry (ESI–MS) and nuclear magnetic resonance (NMR) spectroscopy. The technique introduced in this paper is an efficient method for preparative separation of reserpine and yohimbine from devil pepper radix. It will be beneficial to utilize medicinal materials and also useful for the separation, purification, and pharmacological study of Chinese herbal ingredients.

Słowa kluczowe

EN

pH-zone-refining counter-current chromatography; Rauvolfia verticillata (Lour) Baill; reserpine; yohimbine; alkaloids

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2018
• Tom: Vol. 30, no. 2
• Strony: 81--84
• Opis fizyczny: Bibliogr. 15 poz., rys., tab.

Twórcy

autor

Duan W. J.

• Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, China

autor

Liu Q.

• Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, China
• College of Food Science and Engineering, Shandong Agricultural University, Taian, 271018, China

autor

Zhao R. X.

• Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, China

autor

Mu Y.

• Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, China

autor

Guo L. P.

• National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

autor

Li D. P.

• College of Food Science and Engineering, Shandong Agricultural University, Taian, 271018, China

autor

Wang X.

• Shandong Key Laboratory of TCM Quality Control Technology, Shandong Analysis and Test Center, Shandong Academy of Sciences, China

Bibliografia

• [1] Li, Y. J.; Cao, F. X.; Li, M. Chem. Life. 2015, 2, 258–263.
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• [4] Zhang, B. J.; Peng, L.; Wu, Z. K.; Bao, M. F.; Liu, Y. P.; Cheng, G. G.; Luo, X. D.; Cai, X. H. J. Asian Natur. Prod. research., 2013, 12, 1221–1229.
• [5] Ito, Y. J. Chromatogr. A 2005, 1065, 145–168.
• [6] Ito, Y. J. Chromatogr. A 2013, 1271, 71–85.
• [7] Krystyna, S. W.; Lan, G. Nat. Prod. Rep. 2015, 32, 1556–1561.
• [8] Wang, X.; Liu, Y. Q.; Yang, B.; Geng, Y. L.; Liu, D. H.; Huang, L. Q. Sep. Sci. & Techno. 2011, 9, 1534–1538.
• [9] Fang, L.; Liu, Y. Q.; Yang, B.; Wang, X.; Huang, L. Q. J. Sep. Sci. 2011, 19, 2524–2558.
• [10] Ito, Y.; Ma, Y. J. Chromatogr. A 1996, 753, 1–36.
• [11] Maurya, A.; Gupta, S.; Srivastava, S. K. J. Sep. Sci. 2013, 2, 407–413.
• [12] Ito, Y. J. Chromatogr. A. 2005, 1065, 145–168.
• [13] Fang, L.; Liu, Y. Q.; Yang, B.; Wang, X.; Huang, L. Q. J. Sep. Sci. 2011, 34, 2545–2558.
• [14] Bart, H.; Martin, J. W.; Jan, H. M.; Henk, H. J. Org. Chem. 2011, 21, 8907–8912.
• [15] Rajapaksa, N. S.; McGowan, M. A.; Rienzo, M.; Jacobsen, E. N. Org. Lett. 2013, 3, 706–709.

Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).