Phenolic plant extract enrichment of enzymatically mineralized hydrogels

• Autorzy: Douglas Timothy E.L , Lopez-Heredia Marco A. , Pułczyńska Aleksandra , Łapa Agata , Pietryga Krzysztof , Schaubroeck David , Santos Sónia A.O. , Pais Adriana , Brackman Gilles , Schamphelaere Karel de , Samal Sangram Keshari , Keppler Julia K. , Bauer Jonas L , Chai Feng , Blanchemain Nicolas , Coenye Tom , Pamuła Elżbieta , Skirtach Andre G
• Języki publikacji: EN

Abstrakty

EN

Hydrogel mineralization with calcium phosphate (CaP) and antibacterial activity are desirable for applications in bone regeneration. Mineralization with CaP can be induced using the enzyme alkaline phosphatase (ALP), responsible for CaP formation in bone tissue. Incorporation of polyphenols, plant-derived bactericidal molecules, was hypothesized to provide antibacterial activity and enhance ALP-induced mineralization. Three phenolic rich plant extracts from: (i) green tea, rich in epigallocatechin gallate (EGCG) (herafter referred to as EGCG-rich extract); (ii) pine bark and (iii) rosemary were added to gellan gum (GG) hydrogels and subsequently mineralized using ALP. The phenolic composition of the three extracts used were analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MSn). EGCG-rich extract showed the highest content of phenolic compounds and promoted the highest CaP formation as corroborated by dry mass percentage meassurements and ICP-OES de-termination of mass of elemental Ca and P. All three extracts alone exhibited antibacterial activity in the following order EGCG-rich > PI > RO, respectively. However, extract-loaded and mineralized GG hydro-gels did not exhibit appreciable antibacterial activity by diffusion test. In conclusion, only the EGCG-rich extract promotes ALP-mediated mineralization.

Słowa kluczowe

EN

hydrogel; mineralization; polyphenols; antibacterial; epigallocatechin gallate; gellan gum

• Wydawca: Polish Society for Biomaterials in Cracow
• Czasopismo: Engineering of Biomaterials
• Rocznik: 2019
• Tom: Vol. 22, no. 149
• Strony: 2--9
• Opis fizyczny: Bibliogr. 42 poz., rys., tab., zdj.

Twórcy

autor

Douglas Timothy E.L

• Dept. Molecular Biotechology, Ghent University, Belgium
• Engineering Department, Lancaster University, United Kingdom
• Material Science Institute (MSI), Lancaster University, United Kingdom

autor

Lopez-Heredia Marco A.

• U1008: Controlled Drug Delivery Systems and Biomaterials, Univ. Lille II, France

autor

Pułczyńska Aleksandra

• Dept. Biomaterials and Composites, AGH University of Science and Technology, Kraków, Poland

autor

Łapa Agata

• Dept. Biomaterials and Composites, AGH University of Science and Technology, Kraków, Poland

autor

Pietryga Krzysztof

• Dept. Biomaterials and Composites, AGH University of Science and Technology, Kraków, Poland

autor

Schaubroeck David

• Centre for Microsystems Technology (CMST), imec and Ghent University, Belgium

autor

Santos Sónia A.O.

• CICECO-Aveiro Institute of Materials, Dept. Chemistry, University of Aveiro, Portugal

autor

Pais Adriana

• CICECO-Aveiro Institute of Materials, Dept. Chemistry, University of Aveiro, Portugal

autor

Brackman Gilles

• Laboratory of Pharmaceutical Microbiology, Ghent University, Belgium

autor

Schamphelaere Karel de

• Laboratory for Environmental and Acquatic Ecology, Ghent University, Belgium

autor

Samal Sangram Keshari

• Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Belgium
• Centre for Nano- and Biophotonics, Ghent University, Belgium

autor

Keppler Julia K.

• Dept. Food Technology, Christian-Albrechts-Universität zu Kiel, Germany

autor

Bauer Jonas L

• Dept. Food Technology, Christian-Albrechts-Universität zu Kiel, Germany

autor

Chai Feng

• U1008: Controlled Drug Delivery Systems and Biomaterials, Univ. Lille II, France

autor

Blanchemain Nicolas

• U1008: Controlled Drug Delivery Systems and Biomaterials, Univ. Lille II, France

autor

Coenye Tom

• Laboratory of Pharmaceutical Microbiology, Ghent University, Belgium

autor

Pamuła Elżbieta

• Dept. Biomaterials and Composites, AGH University of Science and Technology, Kraków, Poland

autor

Skirtach Andre G

• Dept. Molecular Biotechology, Ghent University, Belgium
• Centre for Nano- and Biophotonics, Ghent University, Belgium

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Uwagi

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2019).