Computer-aided image analysis for microcapsules' quality assessment

• Autorzy: Przytulska M. , Kulikowski J. L. , Lewińska D. , Grzeczkowicz M. , Kupikowska-Stobba B.

Identyfikatory

DOI

10.1016/j.bbe.2015.05.005

• Języki publikacji: EN

Abstrakty

EN

Application of computer-aided image analysis to quality assessment of inner structure of alginate-polyethersulfone microcapsule is presented. The microcapsules are provided for analysis in the form of optical microscope images of their cross-sections. A specialized computer program APEK makes it possible to measure and calculate a set of morphological parameters describing microcapsules' outer and inner size and shape, as well as the thickness of a polyethersulfone membrane which covers hydrogel microcapsule core. It is described a method of thickness profile of microcapsule's membrane measuring based on skeleton line. Calculations are illustrated by an example. Suggestions concerning future works have been formulated.

Słowa kluczowe

EN

computer aiding; image analysis; alginate polyethersulfone; microcapsules; morphological parameter; thickness profile analysis; size assessment; porosity assessment

PL

wspomaganie komputerowe; analiza obrazu; mikrokapsułka; parametr morfologiczny; ocena wymiaru; ocena porowatości

• Wydawca: Nałęcz Institute of Biocybernetics and Biomedical Engineering of the Polish Academy of Sciences ; Elsevier
• Czasopismo: Biocybernetics and Biomedical Engineering
• Rocznik: 2015
• Tom: Vol. 35, no. 4
• Strony: 342--350
• Opis fizyczny: Bibliogr. 25 poz., rys., tab., wykr.

Twórcy

autor

Przytulska M.

• Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland

autor

Kulikowski J. L.

• Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland

autor

Lewińska D.

• Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland

autor

Grzeczkowicz M.

• Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland

autor

Kupikowska-Stobba B.

• Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, Trojdena 4, 02-109 Warsaw, Poland

Bibliografia

• [1] Chang TMS, Macintosh FC, Mason SG. Semi permeable aqueous microcapsules: I. Preparation and properties. Can J Pharmacol 1966;44:115–28.
• [2] Lewińska D, Bukowski J, Kożuchowski M, Kinasiewicz A, Weryński A. Electrostatic microencapsulation of living cells. Biocybern Biomed Eng 2008;28(2):69–84.
• [3] Camacho FG, Grima EM, Miron AS, Pascual VG, Chisti Y. Carboxymethyl cellulose protects algal cells against hydrodynamic stress. Enzyme Microbiol Technol 2001;29:602–10.
• [4] Uludag H, De Vos P, Tresco P. Technology of mammalian cell encapsulation. Adv Drug Deliv Rev 2000;42:29–64.
• [5] Remunan-Lopez C, Bodmeia R. Mechanical, water uptake and permeability properties of crosslinked chitosan glutamate and alginate films. J Control Release 1997;44: 215–25.
• [6] Usenik P, Vrtovec T, Pernus F, Likar B. Automated tracking and analysis of phospholipids vesicle contours in phase contrast microscopy images. Med Biol Eng Comput 2011;49 (8):957–66.
• [7] Costa NL, Sher P, Mano JF. Liquefied capsules coated with multilayer polyelectrolyte films for cell immobilization. Adv Eng Mater 2011;13(6):B218–24.
• [8] Young J, Poole-Warren LA, Martens PJ. Combining submerged electrospray and UV photopolymerization for production of synthetic hydrogel microspheres for cell encapsulation. Biotechnol Bioeng 2012;109(6):1561–70.
• [9] Gattas-Asfura M, Fraker CA, Stabler CL. Covalent stabilization of alginate hydrogel beads via Staudinger ligation: assessment of poly(ethylene glycol) and alginate cross-linkers. J Biomed Mater Res A 2011;99(1):47–57.
• [10] Lewińska D, Chwojnowski A, Wojciechowski C, Kupikowska-Stobba B, Grzeczkowicz M, Weryński A. Electrostatic droplet generator with 3-coaxial-nozzle head for microencapsulation of living cells in hydrogel covered by synthetic polymer membranes. Sep Sci Technol 2012;47:463–9.
• [11] Lewińska D, Chwojnowski A, Jankowska-Śliwińska J, Weryński A. Microcapsules containing biologically active substances, especially living cells and/or microorganisms, alternatively genetically modified or natural or synthetic for biomedical applications, the way of their manufacturing and the device for this purpose. Patent PL No. 208383; November 15, 2007.
• [12] Smidsrod O, Skjak-Break G. Alginate as immobilization matrix for cells. Trends Biotechnol 1990;8:71–8.
• [13] Chwojnowski A, Wojciechowski C, Dudziński K, Łukowska E, Granicka L. New type of hollow fiber membrane for cell and microorganisms cultivation and encapsulation. Desalination 2009;240:9–13.
• [14] Casanova R, Moukoko D, Pithioux M, Pailler-Mattéi C, Zahouani H, Chabrand P. Temporal evolution of skeletal regenerated tissue: what can mechanical investigation add to biological? Med Biol Eng Comput 2010;48(8):811–9.
• [15] Chwojnowski A, Przytulska M, Wierzbicka D, Kulikowski J, Wojciechowski C. Membranes porosity evaluation by computer-aided analysis of SEM images, a preliminary study. Biocybern Biomed Eng 2012;32(4):65–75.
• [16] Deserno TM, editor. Biomedical image processing. Heidelberg: Springer; 2011 [chapter 6].
• [17] Pitas I. Digital image processing algorithms and applications. A Wiley-Interscience Publication; 2003 [chapters 1, 6, 7].
• [18] Przytulska M, Gierbliński I, Kulikowski JL, Skoczylas K. Quantitative examination of liver tissue ultrasound elastograms. Biocybern Biomed Eng 2011;31(4):75–85.
• [19] Shen L, Rangayyan RM, Desautels JEL. Application of shape analysis to mammographic calcifications. IEEE Trans Med Imaging 1994;13(2):263–74.
• [20] Seul M, O'Gorman L, Samson MJ. Practical algorithms for image analysis. Cambridge University Press; 2000 [chapter 5].
• [21] Piętka D, Dulewicz A, Jaszczak P. Removing artifacts from microscopic images of cytological smears. A shape-based approach. Computer recognition systems. In: Kurzyński M, Puchała E, Woźniak M, Żołnierek A, editors. Advances in soft computing. 2005. pp. 661–9. ISBN: 1434-9922.
• [22] Hoppe A, Wertheim D, Jiang WG, Williams R, Harding K. Interactive image processing system for assessment of cell movement. Med Biol Eng Comput 1999;37(4):419–23.
• [23] Xu D, Balu N, Kerwin WS, Yuan C. Handbook of biomedical image analysis. Segmentation models part B, vol. II. New York: Kluwer Academic/Plenum Publishers; 2005 [chapter 8].
• [24] Saeed K, Tabędzki M, Rybnik M, Adamski M. K3M: a universal algorithm for image skeletonization and a review of thinning techniques. Int J Appl Math Comput Sci 2010;20(2):317–35.
• [25] Russ JC. Image processing handbook. 4th ed. London, Tokyo/Boca Raton, Ann Arbor: CRC Press; 2002 [chapter 8].