First Report on Fusarium brachygibbosum Isolate FIR 16_ITS Isolated from Iraqi Wheat Plant

• Autorzy: Ali Hamdia Z. , Hameed Mahdi S. , Abdulrahman Abdulrahman A. , Saood Hutham M.

Identyfikatory

DOI

10.12911/22998993/118295

• Języki publikacji: EN

Abstrakty

EN

In this study, the Fusarium Brachygibbosum fungus isolate FIR 16_ITS was isolated for the first time from the infected wheat plants in Iraq, during the winter of 2018, in the AL-Tewatha region – 20 km south east of Baghdad. In order to verify the identity of the species of the F. brachygibbosum isolate FIR 16_ITS with the growth symptoms on the wheat plants, the wheat seeds and tissue plants were obtained and used as a source for the re-isolation of the infection agent. F. brachygibbosum isolate FIR 16_ITS was hardly identified using visual approach. However, a molecular technique is important in verifying the species of F. brachygibbosum isolate FIR 16_ITS. The isolate obtained was used for the extraction of DNA and later used for the molecular identification and phylogenetic analyses based on rDNA-internal transcribed region (ITS) primer sets. The authors successfully obtained the amplified products of the ITS-rDNA region, and a sequence analysis indicated that the Fusarium sp. isolate FIR 16_ITS species screened in this study belonged to the F. brachygibbosum species. It was recorded in the NCBI under the following information NCBI; BioSample; Accession no.; SAMN11408139; ID: 11408139 and NCBI; BioProject; Accession no.; PRJNA532637; ID: 679434 (Locus Tag Prefix; E7750).

Słowa kluczowe

EN

phylogenetic analysis; rDNA; Iraq

• Wydawca: Polskie Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology
• Czasopismo: Journal of Ecological Engineering
• Rocznik: 2020
• Tom: Vol. 21, nr 3
• Strony: 81--86
• Opis fizyczny: Bibliogr. 26 poz., rys.

Twórcy

autor

Ali Hamdia Z.

• Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq

autor

Hameed Mahdi S.

• Biotechnology Center, Agriculture Research Directorate, Ministry of Science and Technology, Baghdad, Iraq

autor

Abdulrahman Abdulrahman A.

• Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq

autor

Saood Hutham M.

• Agriculture Research Directorate, Integrated Pest Management Center, Ministry of Science and Technology, Baghdad, Iraq

Bibliografia

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Uwagi

Opracowanie rekordu ze środków MNiSW, umowa Nr 461252 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2020).