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Unconventional yeast in the degradation of hydrocarbons in contaminated soil

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The influence of Yarrowia lipolytica inoculum on biodegradation of hydrocarbons, and changes in microbiota composition in the soil contaminated with petroleum have been investigated. The material under study was contaminated clay soil, containing petroleum-derived substances at approximately 17 000 [mg/kg d. m.]. Microbiological analysis was carried out by the cultivation method and the content of individual hydrocarbons (n-aliphatic, BTEX and PAHs) was determined by the GC/MS method. The largest decrease of oil-derived substances, versus the control sample, was recorded at the beginning of the process. During the first 30 days, the yeast inoculation caused most effectively removal of n-aliphatic hydrocarbons and PAHs (approximately 80% reduction), however, the content of BTEX increased nearly three times. After 60 days of the process, PAHs concentration further decreased (by 40%), concentration of n-aliphatic hydrocarbons decreased a little, however the content of BTEX increased by 10%, compared to the initial concentration. Stimulating the biodegradation process with the yeast inoculum influenced the increase of the bacteria count, mainly Gram-positive, with simultaneous decrease of fungi number.
Rocznik
Strony
87--99
Opis fizyczny
Bibliogr. 24 poz., tab., rys.
Twórcy
  • Chair of Biotechnology and Molecular Biology, University of Opole, pl. Kopernika 11a, 46-020 Opole
  • Chair of Biotechnology and Molecular Biology, University of Opole, pl. Kopernika 11a, 46-020 Opole
Bibliografia
  • [1] HARITASH A.K., KAUSHIK C.P., Biodegradation aspects of polycyclic aromatic hydrocarbons (PAHs). A review, J. Hazard. Mater., 2009, 169 (1–3), 1.
  • [2] LIN X., LI X., SUN T., LI P., ZHOU Q., SUN L., HU X., Changes in microbial populations and enzyme activities during the bioremediation of oil-contaminated soil, Bull. Environ. Contam. Toxicol., 2009, 83 (4), 542.
  • [3] KRZYŚKO-ŁUPICKA T., CIESIELCZUK T., CHWAŁOWSKA M., The biodegradation of petroleum substances in contaminated soil in presence of Fyre-zyme preparation, Acta Sci. Pol. Biotechnol., 2013, (1), 5.
  • [4] ROSIK-DULEWSKA C., KRZYŚKO-ŁUPICKA T., CIESIELCZUK T., KRĘCIDŁO Ł., Hydrogen peroxide as a biodegradation stimulator in remediation processes of soils heavily contaminated with petrochemicals, Pol. J. Chem. Technol., 2015, 17 (2), 17.
  • [5] JUHASZ A.L., NAIDU R., Bioremediation of high molecular weight polycyclic aromatic hydrocarbons. A review the microbal degradation of benzo[a]pyrene, Int. Biodeterior. Biodegradation, 2005, 45, 57.
  • [6] SINGER A.C., VAN DER GAST C.J., THOMPSON I.P., Perspectives and vision for strain selection in bioaugmentation, Trends. Biotechnol., 2005, 23 (2), 74.
  • [7] ŁEBKOWSKA M., ZBOROWSKA E., KARWOWSKA E., MUSZYNSKI A., TABERNACKA A., NAUMCZYK J., JECZALIK M., Bioremediation of soil polluted with fuels by sequential multiple injection of native microorganisms. Field-scale processes in Poland, Ecol. Eng., 2011, 37 (11), 1895.
  • [8] KARAMALIDIS A.K., EVANGELOU A.C., KARABIKA E., KOUKKOU A.I., DRAINAS C., VOUDRIAS E.A., Laboratory scale bioremediation of petroleum-contaminated soil by indigenous microorganisms and added Pseudomonas aeruginosa strain, Spet. Bioresour. Technol., 2010, 101 (16), 6545.
  • [9] BENTO F.M., CAMARGO F.A., OKEKE B.C., FRANKENBERGER W.T., Comparative bioremediation of soils contaminated with diesel oil by natural attenuation, biostimulation and bioaugmentation, Bioresour. Technol., 2005, 96 (9), 1049.
  • [10] MARGESIN R., SCHINNER F., Effect of temperature on oil degradation by psychotrophic yeast in liquid culture and in soil, FEMS Microbiol. Lett., 1997, 24, 243.
  • [11] ROBAK M., BORUCZKOWSKI T., DROŻDŻ W., LAZAR Z., BARANOWSKA M., PRZĄDO D., STEININGER M., Application of the yeasts Yarrowia lipolytica for in-situ bioremediation of soil contaminated with creosote oil, Ochr. Śr., 2011, 33 (2), 27.
  • [12] BARNETT J.A., W PAYNE T.R., YARROW D., Yeast Characteristics and Identification, 3rd Ed., Cambridge University Press, 2001.
  • [13] KOWALCZYK A., MICHNIEWICZ M., ROBAK M., Yarrowia lipolytica in bioremediation: cells entrapped in alginate and their influence on autochthonic microflora and oats germination, Acta Sci. Pol., Biotechn., 2009, 8 (3), 15.
  • [14] BEOPOULOS A., DESFOUGERES T., SABIROVA J., ZINJARDE S., NICAUD J.M., The hydrocarbon degrading oleaginous yeast Yarrowia lipolytica, [In:] K.N. Timmis (Ed.), Handbook of Hydrocarbons and Lipid Biology, Springer-Verlag, Berlin 2010, 2111.
  • [15] JUNG H., AHN Y., CHOI H., KIM I.S., Effects of in-situ ozonation on indigenous microorganisms in diesel contaminated soil: survival and regrowth, Chemosphere, 2005, 61 (7), 923.
  • [16] JUNG H., SOHN K.D., NEPPOLIAN B., CHOI H., Effect of soil organic matter (SOM) and soil texture on the fatality of indigenous microorganisms in integrated ozonation and biodegradation, J. Hazard. Mater., 2008, 150 (3), 809.
  • [17] PITT J., HOCKING A.D., Fungi and Food Spoilage, Academic Press, Australia, 1985.
  • [18] MARGESIN R., HÄMMERLE M., TSCHERKO D., Microbial activity and community composition during bioremediation of diesel-oil-contaminated soil. Effects of hydrocarbon concentration, fertilizers, and incubation time, Microb. Ecol., 2007, 53 (2), 259.
  • [19] KOŁWZAN B., Techniques of petroleum compounds bioremediation and methods of assessment of their effectiveness, Inż. Ekol., 2002, 7, 36.
  • [20] DU W., WAN Y., ZHONG N., FEI J., ZHANG Z., CHEN L., HAO J., Status quo of soil petroleum contamination and evolution of bioremediation, Pet. Sci., 2011, 8, 502.
  • [21] JACQUES R.J., OKEKE B.C., BENTO F.M., TEIXEIRA A.S., PERALBA M.C., CAMARGO F.A., Microbial consortium bioaugmentation of a polycyclic aromatic hydrocarbons contaminated soil, Bioresour. Technol., 2008, 99 (7), 2637.
Typ dokumentu
Bibliografia
Identyfikator YADDA
bwmeta1.element.baztech-d489a6eb-ad89-466f-a6b6-af2b970b66f3
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