New microsatellite multiplex PCR sets for genetic studies of the sterlet sturgeon, Acipenser ruthenus

• Autorzy: Kohlmann K. , Kersten P. , Geßner J. , Onără D. , Taflan E. , Suciu R.

Identyfikatory

DOI

10.14799/ebms285

• Języki publikacji: EN

Abstrakty

EN

Wild populations of the sterlet sturgeon, Acipenser ruthenus, are declining throughout their native ranges. In-depth knowledge of their genetic diversity and structure is urgently needed to enable the identification of management units for conservation purposes. Moreover, genetic markers are required to establish appropriate breeding schemes for supportive stocking programs and to monitor genetic changes in farmed stocks. Therefore, six species-specific, polymorphic microsatellite loci were isolated and arranged into five multiplex PCR sets together with nine loci from other sturgeon species. The diversity of these 15 microsatellites was examined in 67 sterlet individuals (20 farmed in Germany and 47 wild-caught in the Romanian part of the River Danube). The total number of alleles per locus ranged from 3 to 15 with an average of 7.20. The farmed sterlet sturgeon possessed 1 to 7 alleles per locus, with a mean of 3.13; the wild individuals were more variable, with 3 to 15 alleles per locus and a mean of 7.07. Observed heterozygosities ranged from 0 to 0.850 in the farmed individuals, and from 0.064 to 0.957 in the wild individuals. Indications of inbreeding were only found in the wild sterlet sturgeon (FIS=0.062). The genetic differentiation of the two sterlet groups was significant (FST=0.1186). The high sensitivity and discriminatory power of the 15 loci was indicated by the very low overall probability of identity for siblings (PIsib=5.099x10-5) and the high accuracy of self-classification (66 out of the 67 individuals (98.51%) were correctly identified). Thus, these newly developed multiplex PCR sets are a valuable genetic tool for identifying management units for species conservation, sustainable fisheries and aquaculture.

Słowa kluczowe

EN

Acipenser ruthenus; microsatellite; multiplex PCR; species conservation; sustainable fisheries

• Wydawca: University of Warmia and Mazury in Olsztyn, Poland
• Czasopismo: Environmental Biotechnology
• Rocznik: 2017
• Tom: Vol. 13, No. 1
• Strony: 11--17
• Opis fizyczny: Bibliogr. 31 poz., tab.

Twórcy

autor

Kohlmann K.

• Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany; Phone [phone]

autor

Kersten P.

• Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany

autor

Geßner J.

• Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Müggelseedamm 310, 12587 Berlin, Germany

autor

Onără D.

• Danube Delta National Institute, Babadag Str. 165, 820112 Tulcea, Romania

autor

Taflan E.

• Danube Delta National Institute, Babadag Str. 165, 820112 Tulcea, Romania

autor

Suciu R.

• Danube Delta National Institute, Babadag Str. 165, 820112 Tulcea, Romania

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