Kinetics of vinyl acetate biodegradation by Pseudomonas fluorescens PCM 2123

• Autorzy: Gąszczak A. , Bartelmus G. , Greń I. , Janecki D.

Identyfikatory

DOI

10.1515/eces-2018-0033

Warianty tytułu

PL

Kinetyka biodegradacji octanu winylu przez Pseudomonas fluorescens PCM 2123

• Języki publikacji: EN

Abstrakty

EN

The microbial degradation of vinyl acetate (VA) by Pseudomonas fluorescens PCM 2123 strain was studied in both batch and continuous modes. The purpose of the experiments was to determine the kinetic model of the cell growth and biodegradation rate of vinyl acetate (VA), which was the sole carbon and energy source for tested microorganisms. The experiments, carried out in a batch reactor for several initial concentrations of growth substrate in the liquid phase ranging from 18.6 to 373 g_substrate·m⁻³ (g_s·m⁻³) made it possible to choose the kinetic model and to estimate its constants. The Haldane inhibitory model with the values of constants: μ_m = 0.1202 h⁻¹, K_S = 17.195 g_s·m⁻³, K_i = 166.88 g_s·m⁻³ predicted the experimental data with the best accuracy. To set the parameters of maintenance metabolism it was necessary to carry out a series of continuous cultures at different dilution rates (0.05 to 0.072 h⁻¹) and concentrations of VA in the liquid supplied to the chemostat ranging from 30.9 to 123.6 g_s·m⁻³. The obtained data-base enabled to determine the coefficient for maintenance metabolism (m_e = 0.0251 g_substrate· g_{cell dry weight}⁻¹·h⁻¹ (g_s·g_cdw⁻¹·h⁻¹)) as well as the maximal and observed values of yield coefficients, Y_xs^M = 0.463 g_cdw·g_s⁻¹ and (Y_xs)_obs = 0.411 g_cdw·g_s⁻¹, respectively. The developed kinetics was verified by comparison of the computed and obtained in batch experiments profiles of changes in biomass and growth substrate concentrations.

Słowa kluczowe

EN

vinyl acetate; Pseudomonas; kinetics; batch culture; continuous culture

PL

octan winylu; Pseudomonas; kinetyka; hodowla okresowa; hodowle ciągłe

• Wydawca: Towarzystwo Chemii i Inżynierii Ekologicznej
• Czasopismo: Ecological Chemistry and Engineering. S
• Rocznik: 2018
• Tom: Vol. 25, nr 3
• Strony: 487--502
• Opis fizyczny: Bibliogr. 30 poz., rys., wykr.

Twórcy

autor

Gąszczak A.

• Institute of Chemical Engineering, Polish Academy of Sciences, ul. Bałtycka 5, 44-100 Gliwice, Poland

autor

Bartelmus G.

• Institute of Chemical Engineering, Polish Academy of Sciences, ul. Bałtycka 5, 44-100 Gliwice, Poland

autor

Greń I.

• Department of Biochemistry, Faculty of Biology and Environment Protection, University of Silesia, ul. Jagiellońska 28, 40-032 Katowice, Poland

autor

Janecki D.

• Department of Process Engineering, University of Opole, ul. R. Dmowskiego 7-9, 45-365 Opole, Poland

Bibliografia

• [1] Sakunthala M, Sridevi V, Chadana Lakshmi MVV, Vijay Kumar K. A review: the description of three different biological filtration processes and economic evaluation. JECET. 2013;2:91-99. https://www.jecet.org/download_frontend.php?id=63&table=Archive.
• [2] Cheng Y, He H, Yang Ch, Zeng Ch, Li X, Chen H, Yu G. Challenges and solutions for biofiltration of hydrophobic volatile organic compounds. Biotechnol Adv. 2016;34;1091-1102. DOI: 10.1016j.biotechadv.2016.06.007.
• [3] Zdeb M, Lebiocka M. Microbial removal of selected volatile organic compounds from the model landfill gas. Ecol Chem Eng S. 2016;23:215-228. DOI: 10.1515/eces-2016-0014.
• [4] Świerczyńska A, Bohdziewicz J, Puszczało E. Treatment of industrial wastewater in the sequential membrane bioreactor. Ecol Chem Eng S. 2016;23:285-295.DOI: 10.1515/eces-2016-0020.
• [5] Ferdowsi M, Ramirez AA, Jones JP, Heitz M. Elimination of mass transfer and kinetic limited organic pollutants in biofilters: A review. Int Biodeter Biodegr. 2017;119;336-348. DOI: 10.1016/j.ibiod.2016.10.015.
• [6] Federal Institute for Occupational Safety and Health Division for Chemicals and Biocides Regulation Vinyl Acetate - Summary Risk Assessment Report. Dortmund (Germany): 2008. https://echa.europa.eu/documents/10162/6434698/orats_summary_vinylacetate_en.pdf.
• [7] Vinyl Acetate Safe Handling Guide. Washington: Vinyl Acetate Council; 2010. http://msdssearch.dow.com/PublishedLiteratureDOWCOM/dh_0916/0901b803809160d4.pdf?filepath=vam&fromPage=GetDoc.
• [8] http://toxnet.nlm.nih.gov/cgi-bin/sis/search/r?dbs+hsdb:@term+@rn+108-05-4).
• [9] Jakoby WB, Narrod SA. Aldehyde oxidation IV. An aldehyde buffer for growth studies. J Bacteriol. 1959;77:410-413. https://jb.asm.org/content/jb/77/4/410.full.pdf.
• [10] Simon P, Filser JG, Bolt HM. Metabolism of pharmacokinetics of vinyl acetate. Arch Toxicol. 1985;57:191-195. https://link.springer.com/content/pdf/10.1007/BF00290886.pdf.
• [11] Bogdanffy MS, Sarangapani R, Plowchalk DR, Jarabek A, Andersen ME. A biologically based risk assessment for vinyl acetate-induced cancer and noncancer toxicity. Toxicol Sci. 1999;51:19-35. DOI: 10.1093/toxsci/51.1.
• [12] Bogdanffy MS, Taylor ML. Kinetics of nasal carboxylesterase-mediated metabolism of vinyl acetate. Drug Metabol Dispos. 1993;21:1107-1111. http://dmd.aspetjournals.org/content/21/6/1107.short.
• [13] Morris JB, Symanowicz P, Sarangapani R. Regional distribution and kinetics of vinyl acetate hydrolysis in the oral cavity of the rat and mouse. Toxicol Lett. 2002;126:31-99. DOI: 10.1016/S0378-4274(01)00442-8.
• [14] Hatanaka Y, Inoue Y, Murata K, Kimura A. A isolation and characterization of carboxylesterase from vinyl acetate-assimilating bacterium isolated from soil. J Ferment Bioeng. 1989;67:14-19. DOI: 10.1016/0922-338X(89)90079-2.
• [15] Nieder M, Sunarko B, Meyer O. Degradation of vinyl acetate by soil, sewage, sludge, and the newly isolated aerobic bacterium V2. Appl Environ Microbiol. 1990;56:3023-3028. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC184893/.
• [16] Lara-Mayorga I, Duran-Hinojosa U, Arana-Cuenca A, Monroy-Hermosillo O, Ramirez-Vives F. Vinyl acetate degradation by Brevibacillus agri isolates from a slightly aerated methanogenic reactor. Environ Technol. 2010;31:1-6. DOI: 10.1080/09593330903260904.
• [17] Greń I, Gąszczak A, Guzik U, Bartelmus G, Łabużek S. A comparative study of biodegradation of vinyl acetate by environmental strains. Ann Microbiol. 2011;61:257-265. DOI: 10.1007/s13213-010-0130-4.
• [18] Szczyrba E, Greń I, Bartelmus G. Enzymes involved in vinyl acetate decomposition by Pseudomonas fluorescens PCM 2123 strain. Folia Microbiol. 2014;59:99-105. DOI: 10.1007/s12223-013-0268-0.
• [19] Moser A. Bioprocess Technology, Kinetics and Reactors. New York: Springer-Verlag; 1988. DOI: 10.1007/978-1-4613-8748-0.
• [20] EPA On-line Tools for Site Assessment Calculation. http://www3.epa.gov/ceampubl/learn2model/part-two/onsite/esthenry.html.
• [21] Pirt SJ. The maintenance energy of bacteria in growing cultures. Proc R Soc London B. 1965;163: 224-231.
• [22] Schuler ML, Kargi F. Bioprocess Engineering. New Jersey: Prentice Hall, PTR; 2002. ISBN 9780130819086.
• [23] Takeuchi M, Weiss N, Schumann P, Yokota A. Leucobacter komagatae gen. nov., sp. nov., a new aerobic Gram-positive, nonsporulating rod with 2, 4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol. 1996;4:967-971. DOI:10.1099/00207713-46-4-967.
• [24] Bakhshi Z, Najafpour G, Kariminezhad E, Pishgar R, Mousavi N, Taghizade T. Growth kinetic models for phenol biodegradation in a batch culture of Pseudomonas putida. Environ Technol. 2011;32:1835-1841. DOI: 10.1080/09593330.2011.562925.
• [25] Singh RK, Kumar Sh, Kumar S, Kumar A. Biodegradation kinetic studies for the removal of p-cresol from wastewater using Gliomastix indicus MTCC 3869. Biochem Eng J. 2008;40:293-303. DOI: 10.1016/j.bej.2007.12.
• [26] Agarry SE, Solomon BO. Kinetics of batch microbial degradation of phenols by indigenous Pseudomonas fluorescens. IJEST. 2008;5:223-232. http://www.bioline.org.br/pdf?st08026.
• [27] Sherrod PH. Nonlinear Regression Analysis Program (NLREG). Nashville: TN; 2010. www.nlreg.com.
• [28] Nweke CO, Okpokwasili GC. Kinetics of growth and phenol degradation by Pseudomonas species isolated from petroleum refinery wastewater. Int J Biosci. 2014;4:28-37. DOI: 10.12692/ijb/4.7.28-37.
• [29] Molin G. Measurement of the maximum specific growth rate in chemostat of Pseudomonas spp. with different abilities for biofilm formation. Eur J Appl Microbiol Biotechnol. 1983;18:303-307. DOI: 10.1007/BF00500496.
• [30] Hao O, Kim M, Seagren E, Kim H. Kinetics of phenol and chlorophenol utilization by Acinetobacter species. Chemosphere. 2002;46:797-807. DOI: 10.1016/S0045-6535(01)00182-5.