BBAP amplification profiles of apple varieties

• Autorzy: Speváková Ivana , Urbanová Lucia , Kyseľ Matúš , Bilčíková Jana , Farkasová Silvia , Žiarovská Jana

Identyfikatory

DOI

10.5604/01.3001.0015.5233

• Języki publikacji: EN

Abstrakty

EN

Several types of allergies are currently known and are characterized by an exaggerated response of the immune system to substances from various sources called allergens. One of them is a food allergy, which is becoming more common in the population. For this reason, it is necessary to describe the issue from several aspects including genomic variability of plant allergens. The objective of this study was to analyse intraspecific variability of Bet v 1 of 10 different varieties of apple species (Malus domestica Borkh.). BBAP technique for genomic determination of the presence of Bet v 1 homologs at the DNA level was performed. Degenerate primers that anneal a variable and conserved part of PR-10 protein homologues genes were used in the analyse. Amplicons were generated and formed relatively monomorphic profiles, indicating the stability of the given isoforms of Bet v 1 within the selected apple varieties. To evaluate the potential allergenicity of selected varieties further studies on another molecular level such as a comparison of gene expression of the PR-10 family members and their protein expression levels are needed.

Słowa kluczowe

EN

Bet v I; allergen; degenerate primer; apple varietie

PL

Bet v I; alergen; zdegenerowany podkład; odmiana jabłek

• Wydawca: Państwowa Wyższa Szkoła Zawodowa w Tarnowie
• Czasopismo: Science, Technology and Innovation
• Rocznik: 2021
• Tom: Vol. 13, no. 2
• Strony: 1--6
• Opis fizyczny: Bibliogr. 27 poz., rys.

Twórcy

autor

Speváková Ivana

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0002-2669-6398

autor

Urbanová Lucia

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0001-7434-2677

autor

Kyseľ Matúš

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0002-1679-391X

autor

Bilčíková Jana

• AgroBioTech Research Centre, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0003-1738-1113

autor

Farkasová Silvia

• Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

autor

Žiarovská Jana

• Institute of Plant and Environmental Sciences, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic

• https://orcid.org/0000-0002-0005-9729

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