Development and validation of a rapid selective high-throughput LC-MS/MS method for the determination of triclabendazole sulfoxide concentrations in sheep plasma and its use in bioequivalence studies

• Autorzy: Farczádi Lénárd , Dósa Álmos , Melles Orsolya , Vlase Laurian

Identyfikatory

DOI

10.1556/1326.2021.00891

• Języki publikacji: EN

Abstrakty

EN

Triclabendazole is one of the main drugs used to treat liver fluke in livestock. A rapid LC-MS/MS method was developed and validated to determine ovine plasma levels of triclabendazole sulfoxide. A Gemini NX-C18 column was used to achieve analytical separation, with gradient elution of a mobile phase composed of 0.1% formic acid in acetonitril and 0.1% formic acid in water at flow rate of 0.6 mL/min. MRM with positive ESI ionization was used for the detection of triclabendazole sulfoxide (m/z 360.10 from m/z 376.97). Fenbendazole was used as internal standard. Plasma protein precipitation with acetonitrile was used for sample processing. The method was validated with regards to selectivity, linearity (r > 0.9939), within run and between run precision (CV < 8.9%) and accuracy (bias < 8.9%) over the concentration range 1–100 µg/mL plasma. The method developed is simple, selective and can be applied in bioequivalence and bioavailability studies.

Słowa kluczowe

EN

triclabendazole; fenbendazole; LC/MS; ovine; plasma

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2022
• Tom: Vol. 34, no. 2
• Strony: 170--178
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Farczádi Lénárd

• Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Hatieganu”, 8 Victor Babes st., RO-400012, Cluj-Napoca, Romania
• Clinical and Analytical Research Center, Vim Spectrum SRL, 409 Sighisoarei st., RO-547367, TarguMures, Corunca, Romania
• Center for Advanced Medical and Pharmaceutical Research, University of Medicine and Pharmacy, Gheorghe Marinescu st. 38, RO-540142, Targu-Mures, Romania

• https://orcid.org/0000-0002-0834-1614

autor

Dósa Álmos

• Clinical and Analytical Research Center, Vim Spectrum SRL, 409 Sighisoarei st., RO-547367, TarguMures, Corunca, Romania

autor

Melles Orsolya

• Clinical and Analytical Research Center, Vim Spectrum SRL, 409 Sighisoarei st., RO-547367, TarguMures, Corunca, Romania

autor

Vlase Laurian

• Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Hatieganu”, 8 Victor Babes st., RO-400012, Cluj-Napoca, Romania

Bibliografia

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• 3. Mas-Coma, S.; Bargues, M. D.; Valero, M. A. Human fascioliasis infection sources, their diversity, incidence factors, analytical methods and prevention measures. Parasitology 2018, 145, 1665–99.
• 4. Maco, V.; Marcos, L.; Delgado, J.; Herrera, J.; Nestares, J.; Terashima, A. et al. Effcacy and tolerability of two single-day regimens of triclabendazole for fascioliasis in Peruvian children. Rev. Soc. Bras. Med. Trop. 2015, 48(4), 445–53.
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Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).