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Single-cell studies are crucial for gaining knowledge on complexity of intracellular processes. In many cases, carrying researches into cell ingredients must be proceeded by a lysis process. Cell lysis leads to disintegration of the plasma membrane which is the barrier separating cell contents from the environment. However, investigations at the cellular level would not be possible without proper miniaturized tools, which offer many advantages as low reagents consumption, short reaction time, integration, automation or versatility. The goal of this work was to design and develop a microfluidic chip for a chemical cell lysis process. The geometry of a microsystem presented is based on the hydrodynamic focusing of a cell suspension stream. Applying non-denaturing cell lysis buffer enables to analyze released cell ingredients during next steps of investigations.
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Tom
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50--52
Opis fizyczny
Bibliogr. 11 poz., rys.
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- Department of Microbioanalytics, Institute of Biotechnology, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, Poland
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Bibliografia
- [1] Ville, C.A., et al. Biology. 2n d ed. Philadelphia: Saunders, 1998.
- [2] Berg, J.M., et al. Biochemistry. New York: WH Freeman and Company, 2001.
- [3] Brzózka, Z. (red.), Mikrobioanalityka. Warszawa: Oficyna Wydawnicza Politechniki Warszawskiej, 2009.
- [4] Wang, J., “On-chip enzymatic assays.” Electrophoresis 23 (2002): 713–718.
- [5] Brown, R.B., and J. Audet. “Current techniques for single-cell lysis.” Journal of Th e Royal Society Interface 5 (2008): S131–S138.
- [6] Waters, L.C., et al. “Multiple Sample PCR Amplification and Electrophoretic Analysis on a Microchip.” Analytical Chemistry 70 (1998): 5172–5176.
- [7] Han, F., et al. “Fast electrical lysis of cells for capillary electrophoresis.” Analytical Chemistry 75 (2003): 3688--3696.
- [8] Di Carlo, D., K.H. Jeong, and L.P. Lee. “Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation.” Lab on a chip 3 (2003): 287–291.
- [9] Juchniewicz, M., et al. “Bonding-less (B-less) fabrication of polymeric Microsystems.” Microfl uidics and Nanofl uidics 7 (2009): 733–737.
- [10] Mata, A., A.J. Fleischman, and S. Roy. “Characterization of polydimethylsiloxane (PDMS). Properties for Biomedical Micro/Nanosystems.” Biomedical Microdevices 7(4), 2005: 281–293.
- [11] Hofmann, O., P. Niedermann, and A. Manz. “Modular approach to fabrication of three-dimensional microchannel systems in PDMS — application to sheath fl ow microchips.” Lab on a chip 1 (2001): 108–114.
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Bibliografia
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