Preparative separation of steviol glycosides from Stevia rebaudiana bertoni by macroporous resin and preparative HPLC

• Autorzy: Lv X. H. , Kuang P. Q. , Yuan Q. P. , Liang H. , Zheng G. Q.
• Języki publikacji: EN

Abstrakty

EN

In this article, a rapid, novel, and effective method was presented to separate pure steviol glycosides from leaves of Stevia rebaudiana Bertoni, including stevioside, rebaudioside A, and rebaudioside C, using resin-based column chromatography followed by preparative high-performance liquid chromatography under hydrophilic interaction liquid chromatography (HILIC) mode. The separation procedure was first optimized on an analytical HILIC column and then scaled up on a preparative HILIC column using a mobile phase consisting of 83% acetonitrile in water with flow rate of 10 mL•min ^-1 at 25°C. 200 mg of the crude extract containing 68.1% steviol glycosides yielded 79.2 mg stevioside, 9.4 mg rebaudioside C, and 33.7 mg rebaudioside A, with the purities of 97.5%, 96.8%, and 97.2%, respectively. Those products were identified by MS, 1H NMR, and 13C NMR.

Słowa kluczowe

EN

hydrophilic interaction liquid chromatography; macroporous resin; preparative HPLC; separation; steviol glycosides

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2014
• Tom: Vol. 26, no. 1
• Strony: 123--135
• Opis fizyczny: Bibliogr. 27 poz., rys., tab.

Twórcy

autor

Lv X. H.

• Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China

autor

Kuang P. Q.

• Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China

autor

Yuan Q. P.

• Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China

autor

Liang H.

• Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China

autor

Zheng G. Q.

• Beijing University of Chemical Technology State Key Laboratory of Chemical Resource Engineering Beijing 100029 China

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