Effect of sonication reactor geometry on cell disruption and protein release from yeast cells

• Autorzy: Bałdyga J. , Jasińska M. , Dzięgielewska M. , Żochowska M.

Identyfikatory

DOI

10.24425/cpe.2018.124973

• Języki publikacji: EN

Abstrakty

EN

The measured rate of release of intercellular protein from yeast cells by ultrasonication was applied for evaluating the effects of sonication reactor geometry on cell disruption rate and for validation of the simulation method. Disintegration of two strains of Saccharomyces cerevisiae has been investigated experimentally using a batch sonication reactor equipped with a horn type sonicator and an ultrasonic processor operating at the ultrasound frequency of 20 kHz. The results have shown that the rate of release of protein is directly proportional to the frequency of the emitter surface and the square of the amplitude of oscillations and strongly depends on the sonication reactor geometry. The model based on the Helmholtz equation has been used to predict spatial distribution of acoustic pressure in the sonication reactor. Effects of suspension volume, horn tip position, vessel diameter and amplitude of ultrasound waves on the spatial distribution of pressure amplitude have been simulated. A strong correlation between the rate of protein release and the magnitude of acoustic pressure and its spatial distribution has been observed. This shows that modeling of acoustic pressure is useful for optimization of sonication reactor geometry.

Słowa kluczowe

EN

cell disruption; protein release; Saccharomyces cerevisiae ultrasonication yeast

PL

dezintegracja komórek; uwalnianie białka; drożdże

• Wydawca: Komitet Inżynierii Chemicznej i Procesowej Polskiej Akademii Nauk
• Czasopismo: Chemical and Process Engineering
• Rocznik: 2018
• Tom: Vol. 39, nr 4
• Strony: 475--–489
• Opis fizyczny: Bibliogr. 21 poz.

Twórcy

autor

Bałdyga J.

autor

Jasińska M.

autor

Dzięgielewska M.

autor

Żochowska M.

Bibliografia

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Uwagi

PL

Opracowanie rekordu w ramach umowy 509/P-DUN/2018 ze środków MNiSW przeznaczonych na działalność upowszechniającą naukę (2018).