Micro-sample analysis of capsaicin by liquid chromatography-tandem mass spectrometry assay and application to oral absorption study

• Autorzy: Choi Y. , Shin B. S. , Kim M. G. , Shin S. W. , Choo H. W. , Kim T.H. , Jang H. C , Yoo S. D.
• Języki publikacji: EN

Abstrakty

EN

Capsaicin has been reported to exhibit an inhibitory effect on the P-glycoprotein (P-gp) function in vitro. To investigate its concentration-dependent effect in vivo, a sensitive assay that can characterize the absorption and disposition of capsaicin needs to be developed. This study reports the development of a sensitive LC-MS/MS assay for the determination of capsaicin in mouse plasma. The sample pretreatment involved a one-step extraction of 20 μL plasma with t-butyl methyl ether. Separations were achieved on a C18 column and the detection was performed on an LC-ESI-MS/MS by multiple reaction monitoring. The assay was linear over a wide concentration range from 0.325 to 650 ng mL^-1 (r > 0.999), with a LLOQ of 0.325 ng mL^-1. The developed method was applied to i.v. (dose 0.325 and 0.65 mg kg) and oral absorption (dose 40 mg kg) studies in mice. After i.v. injection, the t1/2,λz, Vz and CLs ranged from 0.13–0.16 h, 127.6–141.8 mL, and 547.3–775.4 mL/h, respectively. After oral administration, a secondary peak was observed and the terminal half-life was prolonged (1.51 h). Capsaicin was poorly absorbed, with the absolute oral bioavailability (F) ranging from 1.02% to 1.56%. The developed assay may be useful in studies where sample volumes are limited.

Słowa kluczowe

EN

capsaicin; LC-MS/MS; bioavailability; pharmacokinetics

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2013
• Tom: Vol. 25, no. 2
• Strony: 363--376
• Opis fizyczny: Bibliogr. 17 poz., rys., tab.

Twórcy

autor

Choi Y.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Shin B. S.

• Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea

autor

Kim M. G.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Shin S. W.

autor

Choo H. W.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Kim T.H.

• Catholic University of Daegu College of Pharmacy Gyeongsan-si, Gyeongbuk Korea

autor

Jang H. C

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

autor

Yoo S. D.

• Sungkyunkwan University School of Pharmacy Suwon, Gyeonggi-do Korea

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