Obtaining Temperature-Resistant Sugar Beet Lines (Beta vulgaris L.)

• Autorzy: Kliachenko Oksana , Prysiazhniuk Larysa , Bokiy Olena , Syplyva Natalia , Melnyk Serhii

Identyfikatory

DOI

10.12912/27197050/154913

• Języki publikacji: EN

Abstrakty

EN

This study aimed to investigate the sugar beet genotypes for resistance to hyperthermia and obtain temperatureresistant lines. Nine hybrids and one variety of sugar beet were screened. Cotyledonary leaves and hypocotyls isolated from aseptic seedlings were used for induction of callus and subsequent subcultivation. To create hyperthermic conditions, the callus lines were maintained in thermostats at temperatures of +27 °C, +41 °C, +45 °C, and +47 °C. The effect of high temperatures on the callus tissue was assessed by the specific callus diameter index. The free proline was detected with chromatography. As result of callus tissue exposed to temperatures of +41 °C, 45 °C and 47 °C, on the 9th day of cultivation at high temperatures, significant differences were observed in the size and colouration of the callus tissues. At a moderate temperature (+41 °C), the growth of the callus mass was somewhat higher compared to the control. At a high temperature (+45 °C), the intensity of the growth processes decreased and ceased at a temperature of +47 °C. After transfer and subsequent cultivation of callus tissues in regeneration medium MSR – Murashige and Skoog medium for regeneration, all genotypes demonstrated the formation of morphological structures that initiated the formation of regenerated plants. The number of regenerated plants largely fluctuated over temperatures and almost was not related to genotypes. Consequently, the temperature-resistant lines obtained as a result of extreme heat treatment, differ in terms of the specific diameter of callus.

Słowa kluczowe

EN

specific diameter of calluses; proline; hypothermia; cell selection; in vitro

• Wydawca: Lubelski Oddział Polskiego Towarzystwo Inżynierii Ekologicznej ; Lublin University of Technology ; WNGB Wydawnictwo Naukowe
• Czasopismo: Ecological Engineering & Environmental Technology
• Rocznik: 2023
• Tom: Vol. 24, iss. 1
• Strony: 22--28
• Opis fizyczny: Bibliogr. 19 poz., rys., tab.

Twórcy

autor

Kliachenko Oksana

• National University of Life and Environmental Sciences of Ukraine, 15 Heroiv Oborony St., Kyiv, 03041, Ukraine

• https://orcid.org/0000-0002-4087-4082

autor

Prysiazhniuk Larysa

• Ukrainian Institute for Plant Variety Examination, 15 Henerala Rodimtseva St., 03041, Kyiv, Ukraine

• https://orcid.org/0000-0003-4388-0485

autor

Bokiy Olena

• Institute of Food Resourses of National Academy of Agrarian Sciences of Ukraine, 4-a Yevgen Sverstiuk St., Kyiv, Ukraine

• https://orcid.org/0000-0001-9916-1856

autor

Syplyva Natalia

• Ukrainian Institute for Plant Variety Examination, 15 Henerala Rodimtseva St., 03041, Kyiv, Ukraine

• https://orcid.org/0000-0003-0921-6361

autor

Melnyk Serhii

• Ukrainian Institute for Plant Variety Examination, 15 Henerala Rodimtseva St., 03041, Kyiv, Ukraine

• https://orcid.org/0000-0002-5514-5819

Bibliografia

• 1. Arrieta M., Willems G., DePessemier J., Colas I., Burkholz A., Darracq A., Ramsay L. 2021. The effect of heat stress on sugar beet recombination. Theoretical and Applied Genetics, 134(1), 81–93. https://doi.org/10.1007/s00122-020-03683-0
• 2. Belan N.F., Abdurahmanova Z.N. 1969. The separation of photosynthesis products by thin layer chromatography. DAN TajSSR, 10, 61−65. (in Russian)
• 3. Butenko R.G. 1999. Biology of Cells of Higher Plants in vitro and Biotechnology on Their Basis. FBK-Press, Moscow. (in Russian)
• 4. Butenko R.G., Atanassov A.I., Urmantseva V.V. 1972. Some features of sugar beet tissue cultures. Phytomorphology, 22, 140–143.
• 5. Checheneva T.N. 2006. Variability of cereals in vitro and in the process of regeneration of plants. Physiology and Biochemistry of Cultural Plants, 38(2), 163–175. (in Russian)
• 6. Dubrovna O.V., Morhun B.V. 2009. Cell breeding of wheat for resistance to stressful environmental factors. Physiology and Biochemistry of Cultivated Plants, 6, 463–475. (in Russian)
• 7. Golovko T., Tabalenkova G. 2014. Pigments and productivity of crop plants. In: Photosynthetic Pigments: Chemical Structure, Biological Function and Ecology. Syktyvkar.
• 8. Kliachenko O.L., Prysiazhniuk L.M. 2014. Study on the allelic state of microsatellite loci of sugar beet (Beta vulgaris L.). Living and bioinert systems, 38, 125−137. (in Russian)
• 9. Kliachenko O.L., Prysiazhniuk L.M. 2016. Differentiation and identification of various genotypes of sugar beet (Beta vulgaris L.) using DNA markers. In: Scientific Reports of the NUBNRU of Ukraine. (in Ukrainian)
• 10. Kolupaev Yu.E., Vayner A.A., Yastreb T.O. 2014. Prolin: physiological functions and regulation of content in plants under stressful conditions. In: Bulletin of the Kharkiv National Agrarian University: Biology series, 2(32), 6−22. (in Russian.)
• 11. Kucherenko L.A., Madumache R.P., Guzhov Yu.L. 1991. To the methodology for the determination of the weight of callus tissues in the process of cultivation. Agricultural Biology, 3, 84–86. (in Russian)
• 12. Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant., 15, 473–497.
• 13. Philanim W. S., Kumar A., Shettigar N. 2022. Biotechnological Approaches in Sugar Beet Development. In Sugar Beet Cultivation, Management and Processing Springer, Singapore, 75–89. https://doi.org/10.1007/978-981-19-2730-0_5
• 14. Pomeroy M.K., Mudd J.B. 1987. Chilling sensitivity of cucumber cotyledon protoplast and seedling. Plant Phisiol., 84(3), 677–681.
• 15. Shevyreva G.A., Zhestokova I.M., Trofimova M.S. 2007. Immunolocalization of PIP aquaporins in protoplasts from the suspension culture of mesophyll of sugar beet in isosmotic conditions and under osmotic stress. Plant Physiology, 57(3), 356–364.
• 16. Stevanato P., Chiodi C., Broccanello C., Concheri G., Biancardi E., Pavli O., Skaracis G. 2019. Sustainability of the sugar beet crop. Sugar Tech, 21(5), 703–716. https://doi.org/10.1007/s12355-019-00734-9
• 17. Titok V.V. 2008. Bioenergy concept of heterosis. Molecular and Applied genetics, 8, 81–93. (in Russian)
• 18. Taleghani D., Rajabi A., Hemayati S. S., Saremirad A. 2022. Improvement and selection for droughttolerant sugar beet (Beta vulgaris L.) pollinator lines. Results in Engineering, 13, 100367. https://doi.org/10.1016/j.rineng.2022.100367
• 19. Zhuchenko A.A. 2008. Adaptive Crop Production (Ecological and Genetic Bases): Theory and Practice. V. 1. Agrorus, Moscow. (in Russian)

Uwagi

Opracowanie rekordu ze środków MEiN, umowa nr SONP/SP/546092/2022 w ramach programu "Społeczna odpowiedzialność nauki" - moduł: Popularyzacja nauki i promocja sportu (2022-2023).