Pre-probiotic effects of different bacterial species in aquaculture : behavioral, hematological and oxidative stress responses

• Autorzy: Alak Gonca , Kotan Recep , Uçar Arzu , Parlak Veysel , Atamanalp Muhammed

Identyfikatory

DOI

10.26881/oahs-2022.2.01

• Języki publikacji: EN

Abstrakty

EN

There is very limited aquaculture research on candidate probiotics and their effects on fish physiology. In this study, acute applications of four different molecularly identified bacterial species – Brevibacillus brevis FD-1 (A), Pseudomonas fluorescens FDG-37 (B), Bacillus sphaericus FD-48 (C), and B. amyloliquefaciens TV-17C (D), with potential in aquaculture, were tested in rainbow trout (Oncorhynchus mykiss) under static conditions. Physiological changes in blood tissue [hematological indices: erythrocyte count (RBC), leukocyte count (WBC), hemoglobin (Hb), hematocrit (Hct), platelet count (PLT), mean cell hemoglobin concentration (MCHC), mean cell hemoglobin (MCH), and mean cell volume (MCV)], oxidative stress responses in liver and gill tissues [malondialdehyde (MDA) level, antioxidant enzyme activities: superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GP_X), glutathione reductase (GR), glutathione-S-transferase (GST), glucose-6-phosphate dehydrogenase (G6PD)] and acetylcholinesterase (AChE) activity in brain tissue (as neurotoxic biomarker) were investigated. Additionally, behavioral differences were recorded by measuring swimming performance to support neurotoxic findings in all treatment groups. The LC₅₀24 value of FDG-37 strain was determined through analysis as 1.0 × 10⁸CFU ml^-1. Inhibition of enzyme activity, increase in the MDA level, as well as significant differences in hematological indices and swimming performance were determined in rainbow trout treated with B compared to control and other bacterial groups in gills. The potential for using group FD-48 and TV-17C bacterial strains as probiotics in aquaculture is more pertinent when considering the research findings and water quality parameters.

Słowa kluczowe

EN

fisheries; ecosystem management; probiotic; oxidative stress; LC50

• Wydawca: Institute of Oceanography University of Gdańsk
• Czasopismo: Oceanological and Hydrobiological Studies
• Rocznik: 2022
• Tom: Vol. 51, No. 2
• Strony: 133--142
• Opis fizyczny: Bibliogr. 23 poz., rys., tab.

Twórcy

autor

Alak Gonca

• Department of Seafood Processing Technology, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey

autor

Kotan Recep

• Department of Plant Protection, Faculty of Agriculture, Ataturk University, TR-25240 Erzurum, Turkey

autor

Uçar Arzu

• Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey

autor

Parlak Veysel

• Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey

autor

Atamanalp Muhammed

• Department of Aquaculture, Faculty of Fisheries, Ataturk University, TR-25030 Erzurum, Turkey

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