Efficient method for extraction and isolation of shionone from Aster tataricus L. f. by supercritical fluid extraction and high-speed counter-current chromatography

• Autorzy: Wang D , Bai A , Lin X. , Fang L. , Shu X. , Shi X. , Sun Q. , Wang X.
• Języki publikacji: EN

Abstrakty

EN

Supercritical fluid extraction (SFE) was used to extract shionone from Aster tataricus L. f. The effect of various parameters, i.e., temperature, pressure and sample particle size on yield was investigated with an analytical-scale SFE system to find the optimal conditions. The process was then scaled up by 50 times with a preparative SFE system under the optimized conditions of temperature 40 °C, pressure 30 MPa, and a sample particle size of 40–60 mesh. Then preparative high-speed counter-current chromatography was successfully used for isolation and purification of shionone from the SFE extract with a two-phase solvent system composed of n-hexane-methanol (2:1, volume ratio). The separation produced a total of 75 mg of shionone from 500 mg of the crude extract in one step separation with the purity of 98.7%, respectively, as determined by high-performance liquid chromatography (HPLC) and 92% recovery. The structure of shionone was identified by electrospray ionization-mass spectrometry (ESI-MS), hydrogen-1 nuclear magnetic resonance (1H-NMR), and carbon-13 nuclear magnetic resonance (13C-NMR).

Słowa kluczowe

EN

supercritical fluid extraction (SFE); high-speed counter-current chromatography; Shionone; Aster tataricus L. f.

• Wydawca: University of Silesia in Katowice ; Akademiai Kiado
• Czasopismo: Acta Chromatographica
• Rocznik: 2012
• Tom: Vol. 24, no. 4
• Strony: 615--625
• Opis fizyczny: Bibliogr. 16 poz., rys., tab.

Twórcy

autor

Wang D

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

autor

Bai A

• inan Municipal Center For Disease Control and Prevention Jinan 250001 P. R. China

autor

Lin X.

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

autor

Fang L.

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

autor

Shu X.

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

autor

Shi X.

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

autor

Sun Q.

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

autor

Wang X.

• Shandong Academy of Sciences Shandong Analysis and Test Center 19 Keyuan Street Jinan 250014 P. R. China

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